Journal ArticleDOI
Chromosome mapping and expression of a putative testis-determining gene in mouse.
TLDR
Polymerase chain reaction analysis suggests both Y loci are transcribed in adult testes, and mYfin sequences have been duplicated several times in the mouse, although they are not duplicated in humans.Abstract:
Isolation and mapping of a mouse complementary DNA sequence (mouse Y-finger) encoding a multiple, potential zinc-binding, finger protein homologous to the candidate human testis-determining factor gene is reported. Four similar sequences were identified in Hind III-digested mouse genomic DNA. Two (7.2 and 2.0 kb) were mapped to the Y chromosome. Only the 2.0-kb fragment, however, was correlated with testis determination. Polymerase chain reaction analysis suggests both Y loci are transcribed in adult testes. A 3.6-kb fragment was mapped to the X chromosome between the T16H and T6R1 translocation breakpoints, and a fourth (6.0 kb) was mapped to chromosome 10. Hence, mYfin sequences have been duplicated several times in the mouse, although they are not duplicated in humans.read more
Citations
More filters
Journal ArticleDOI
Male development of chromosomally female mice transgenic for Sry
TL;DR: It is shown that Sry on a 14-kilobase genomic DNA fragment is sufficient to induce testis differentiation and subsequent male development when introduced into chromosomally female mouse embryos.
Journal ArticleDOI
A gene mapping to the sex-determining region of the mouse Y chromosome is a member of a novel family of embryonically expressed genes
John Gubbay,Jérôme Collignon,Peter Koopman,Blanche Capel,Androulla Economou,Andrea Münsterberg,Nigel Vivian,Peter N. Goodfellow,Robin Lovell-Badge +8 more
TL;DR: A gene mapping to the sex-determining region of the mouse Y chromosome is deleted in a line of XY female mice mutant for Tdy, and is expressed at a stage during male gonadal development consistent with its having a role in testis determination.
Journal ArticleDOI
The origin and efficient derivation of embryonic stem cells in the mouse
F. A. Brook,Richard L. Gardner +1 more
TL;DR: By explanting tissues isolated microsurgically from implanting strain 129 mouse blastocysts individually on STO feeder cells, it is established that embryonic stem (ES) cells originate from the epiblast (primitive ectoderm).
Journal ArticleDOI
Evolution of the Zfx and Zfy genes: rates and interdependence between the genes.
Pekka Pamilo,Néstor O. Bianchi +1 more
TL;DR: A phylogenetic analysis of sex-chromosomal zinc-finger genes (Zfx and Zfy) indicates that the genes have not evolved completely independently since their initial separation.
Journal ArticleDOI
Mouse embryonic germ (EG) cell lines : transmission through the germline and differences in the methylation imprint of insulin-like growth factor 2 receptor (Igf2r) gene compared with embryonic stem (ES) cell lines
TL;DR: The derivation of multiple embryonic germ cell lines from 8.5 days post coitum embryos of C57BL/6 inbred mice are reported, showing that pluripotent cell lines capable of forming teratocarcinomas and coat color chimeras can be established from primordial germ cells of 8.0 days p.c.
References
More filters
Journal ArticleDOI
Most classical Mus musculus domesticus laboratory mouse strains carry a Mus musculus musculus Y chromosome.
TL;DR: Using a Y-specific genomic DNA probe obtained from a flow-sorted mouse Y-chromosome library, this work has examined the RFLPs in 10 newly established mouse lines of the European semispecies, and identified two variant forms of the Y chromosome, each of which is characteristic of one of the sem ispecies.
Journal Article
The William Allan memorial award address: X-chromosome inactivation and the location and expression of X-linked genes.
TL;DR: Genes lies au chromosome X: identite et localisation, expression, inactivation, reactivation, and so on.
Journal ArticleDOI
Meitoic crossing-over between the X and Y chromosomes of male mice carrying the sex-reversing (Sxr) factor
TL;DR: Evidence is presented here that X/Y Sxr mice have a single abnormal Y chromosome possessing an additional dark-staining distal body, and during meiosis, this body is transferred by crossing-over from one chromatid of the Y to one chromatin of the X.
Journal ArticleDOI
Spermatogenic failure in male mice lacking H-Y antigen.
TL;DR: It is shown that H–Y-negative male mice, in losing the genetic information that encodes H-Y, have also lost genetic information required for spermatogenesis, and this result identifies a gene on the mouse Y,inct from the testis-determining gene, which is necessary for sarcolysis, and raises the intriguing possibility that the product of this ‘spermatogenic gene’ is H–y antigen.
Journal ArticleDOI
Human chromosome–specific DNA libraries: Construction and availability
M. A. Van Dilla,Larry L. Deaven,Kevin L. Albright,N. A. Allen,M. R. Aubuchon,Marty F. Bartholdi,Nancy C. Brown,E.W. Campbell,Anthony V. Carrano,Lynn M. Clark,L. S. Cram,B D Crawford,J. C. Fuscoe,Joe W. Gray,C.E. Hildebrand,Paul J. Jackson,James H. Jett,Jonathan L. Longmire,C. R. Lozes,M. L. Luedemann,John C. Martin,J. S. McNinch,Linda Meincke,Mortimer L. Mendelsohn,J. Meyne,Robert K. Moyzis,A C Munk,J. Perlman,D. Peters,A. J. Silva,Barbara J. Trask +30 more
TL;DR: The specific aim of Phase I of the project is the production of complete digest libraries from each of the 24 human chromosomal types purified by flow sorting, and the construction of libraries with large inserts in a more ad vanced, recently developed bacteriophage vector.