Journal ArticleDOI
Clathrin-coated vesicles: Isolation, dissociation and factor-dependent reassociation of clathrin baskets
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TLDR
The clathrin coat is a labile structure that can be solubilized by nondenaturing treatments, and baskets can be reformed from the extracted material.About:
This article is published in Cell.The article was published on 1979-02-01. It has received 404 citations till now. The article focuses on the topics: Clathrin binding & Clathrin coat.read more
Citations
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Coated pits, coated vesicles, and receptor-mediated endocytosis
TL;DR: It is now recognised that receptor-mediated endocytosis has a fundamental role in the growth, nutrition and differentiation of animal cells.
Journal ArticleDOI
Endocytosis and the recycling of plasma membrane.
TL;DR: This article centers on the properties and dynamics of the endocytic vacuole membrane and stresses observations on cultured mouse macrophages with which the authors are most familier.
Journal ArticleDOI
Beta-arrestin acts as a clathrin adaptor in endocytosis of the beta2-adrenergic receptor.
Oscar B. Goodman,Jason G. Krupnick,Francesca Santini,Vsevolod V. Gurevich,Vsevolod V. Gurevich,Raymond B. Penn,Alison W. Gagnon,James H. Keen,Jeffrey L. Benovic +8 more
TL;DR: The results show that β-arrestin functions as an adaptor in the receptor-mediated endocytosis pathway, and suggest a general mechanism for regulating the trafficking of G-protein-coupled receptors.
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Hypertonic media inhibit receptor-mediated endocytosis by blocking clathrin-coated pit formation.
John E. Heuser,R. G. W. Anderson +1 more
TL;DR: In spite of their seeming unrelatedness, both K+ depletion and hypertonic treatment cause coated pits to disappear, and both induce abnormal clathrin polymerization into empty microcages, which suggests that in both cases, an abnormal formation of microc cages inhibits endocytosis.
Journal ArticleDOI
Functional partnership between amphiphysin and dynamin in clathrin-mediated endocytosis.
TL;DR: Results show that amphiphysin binds lipid bilayers, indicate a potential function for amphiphYSin in the changes in bilayer curvature that accompany vesicle budding, and imply a close functional partnership between amphiphisin and dynamin in endocytosis.
References
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A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
Journal ArticleDOI
The fluid mosaic model of the structure of cell membranes.
S. J. Singer,Garth L. Nicolson +1 more
TL;DR: Results strongly indicate that the bivalent antibodies produce an aggregation of the surface immunoglobulin molecules in the plane of the membrane, which can occur only if the immunoglOBulin molecules are free to diffuse in the membrane.
Book ChapterDOI
Assay of inorganic phosphate, total phosphate and phosphatase
TL;DR: The method is about seven times as sensitive as the Fiske–SubbaRow procedure and involves less pipetting, but it is not very satisfactory for determining inorganic phosphate if labile phosphate esters are present in large excess.
Journal ArticleDOI
Evidence for recycling of synaptic vesicle membrane during transmitter release at the frog neuromuscular junction
John E. Heuser,Thomas S. Reese +1 more
TL;DR: During stimulation the intracellular compartments of this synapse change shape and take up extracellular protein in a manner which indicates that synaptic vesicle membrane added to the surface during exocytosis is retrieved by coated vesicles and recycled into new synaptic vESicles by way of intermediate cisternae.