Clinical features and virological analysis of a case of Middle East respiratory syndrome coronavirus infection
Christian Drosten,M Seilmaier,Victor M. Corman,W. J. Hartmann,Gregor Scheible,Stefan Sack,Wolfgang Guggemos,René Kallies,Doreen Muth,Sandra Junglen,Marcel A. Müller,Walter Haas,Hana Guberina,Tim Röhnisch,Monika Schmid-Wendtner,Souhaib Aldabbagh,Ulf Dittmer,Hermann Gold,Petra Graf,Frank Bonin,Andrew Rambaut,Andrew Rambaut,Clemens M. Wendtner +22 more
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TLDR
The first complete viral load profile in a case of MERS-CoV infection is provided, which might have shedding patterns that are different from those of severe acute respiratory syndrome and so might need alternative diagnostic approaches.Abstract:
Summary Background The Middle East respiratory syndrome coronavirus (MERS-CoV) is an emerging virus involved in cases and case clusters of severe acute respiratory infection in the Arabian Peninsula, T unisia, Morocco, France, Italy, Germany, and the UK. We provide a full description of a fatal case of MERS-CoV infection and associated phylogenetic analyses. Methods We report data for a patient who was admitted to the Klinikum Schwabing (Munich, Germany) for severe acute respiratory infection. We did diagnostic RT -PCR and indirect immunofl uorescence. From time of diagnosis, respiratory, faecal, and urine samples were obtained for virus quantifi cation. We constructed a maximum likelihood tree of the fi ve available complete MERS-CoV genomes.read more
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Comparative Evaluation of Three Homogenization Methods for Isolating Middle East Respiratory Syndrome Coronavirus Nucleic Acids From Sputum Samples for Real-Time Reverse Transcription PCR.
TL;DR: The PK-DNase method is suitable for homogenizing sputum samples prior to RNA extraction and differences in ranges of Ct values obtained by the PBS and NALC methods differed significantly from the mean control Ct.
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MERS-CoV recombination: implications about the reservoir and potential for adaptation
Gytis Dudas,Andrew Rambaut +1 more
TL;DR: The phylogenetic methods and simulations are used to show that MERS-CoV genome has likely undergone numerous recombinations recently, which implies frequent co-infection with distinct lineages of MERS -CoV, probably in camels given the current understanding of MSPS epidemiology.
Journal ArticleDOI
MERS-CoV at the animal-human interface: inputs on exposure pathways from an expert-opinion elicitation.
Anna L. Funk,Flavie Goutard,Eve Miguel,Mathieu Bourgarel,Véronique Chevalier,Bernard Faye,J. S. Malik Peiris,Maria D. Van Kerkhove,François Roger +8 more
TL;DR: The results of this study are consistent with available, yet very limited, published data regarding the potential pathways of transmission of MERS-CoV at the animal–human interface and identify key knowledge gaps.
Journal ArticleDOI
Middle East Respiratory Syndrome (MERS).
TL;DR: E Epidemiological and other data suggest that the source of most primary cases is exposure to camels, and person-to-person transmission occurs in household and health care settings, although sustained and efficient person- to- person transmission has not been observed.
Journal ArticleDOI
Systemic innate and adaptive immune responses to SARS-CoV-2 as it relates to other coronaviruses.
TL;DR: In this review, what is currently known about the systemic immune response to SARS-CoV-2 and potential immunotherapeutic approaches are summarized.
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