Clinical features and virological analysis of a case of Middle East respiratory syndrome coronavirus infection
Christian Drosten,M Seilmaier,Victor M. Corman,W. J. Hartmann,Gregor Scheible,Stefan Sack,Wolfgang Guggemos,René Kallies,Doreen Muth,Sandra Junglen,Marcel A. Müller,Walter Haas,Hana Guberina,Tim Röhnisch,Monika Schmid-Wendtner,Souhaib Aldabbagh,Ulf Dittmer,Hermann Gold,Petra Graf,Frank Bonin,Andrew Rambaut,Andrew Rambaut,Clemens M. Wendtner +22 more
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TLDR
The first complete viral load profile in a case of MERS-CoV infection is provided, which might have shedding patterns that are different from those of severe acute respiratory syndrome and so might need alternative diagnostic approaches.Abstract:
Summary Background The Middle East respiratory syndrome coronavirus (MERS-CoV) is an emerging virus involved in cases and case clusters of severe acute respiratory infection in the Arabian Peninsula, T unisia, Morocco, France, Italy, Germany, and the UK. We provide a full description of a fatal case of MERS-CoV infection and associated phylogenetic analyses. Methods We report data for a patient who was admitted to the Klinikum Schwabing (Munich, Germany) for severe acute respiratory infection. We did diagnostic RT -PCR and indirect immunofl uorescence. From time of diagnosis, respiratory, faecal, and urine samples were obtained for virus quantifi cation. We constructed a maximum likelihood tree of the fi ve available complete MERS-CoV genomes.read more
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Journal ArticleDOI
Active Replication of Middle East Respiratory Syndrome Coronavirus and Aberrant Induction of Inflammatory Cytokines and Chemokines in Human Macrophages: Implications for Pathogenesis
Jie Zhou,Hin Chu,Cun Li,Bosco Ho-Yin Wong,Zhongshan Cheng,Vincent Kwok-Man Poon,Tianhao Sun,Candy Choi-Yi Lau,Kenneth K. Y. Wong,Jimmy Yu Wai Chan,Jasper Fuk-Woo Chan,Kelvin K. W. To,Kwok-Hung Chan,Bo-Jian Zheng,Kwok-Yung Yuen +14 more
TL;DR: It is conclusively showed that MERS-CoV can establish a productive infection in human macrophages and the aberrant induction of inflammatory cytokines/chemokines could be important in the disease pathogenesis.
Journal ArticleDOI
Interhuman transmissibility of Middle East respiratory syndrome coronavirus: estimation of pandemic risk
TL;DR: The analysis suggests that MERS-CoV does not yet have pandemic potential, and recommends enhanced surveillance, active contact tracing, and vigorous searches for the MERS -CoV animal hosts and transmission routes to human beings.
Journal ArticleDOI
Transmission of MERS-Coronavirus in Household Contacts
Christian Drosten,Benjamin Meyer,Marcel A. Müller,Victor M. Corman,Malak Almasri,Raheela Hossain,Hosam Madani,Andrea Sieberg,Berend Jan Bosch,Erik Lattwein,Raafat F. Alhakeem,Abdullah M. Assiri,Waleed H. Hajomar,Ali Albarrak,Jaffar A. Al-Tawfiq,Alimuddin Zumla,Ziad A. Memish +16 more
TL;DR: The rate of secondary transmission among household contacts of patients with MERS-CoV infection has been approximately 5%, and the data provide insight into the rate of subclinical transmission of MSPS coronavirus in the home.
Journal ArticleDOI
Clinicopathologic, Immunohistochemical, and Ultrastructural Findings of a Fatal Case of Middle East Respiratory Syndrome Coronavirus Infection in the United Arab Emirates, April 2014
Dianna Ng,Farida A L Hosani,M. Kelly Keating,Susan I. Gerber,Tara Jones,Maureen G. Metcalfe,Suxiang Tong,Ying Tao,Negar N. Alami,Lia M. Haynes,Mowafaq Ali Mutei,Laila AbdelWareth,Timothy M. Uyeki,David L. Swerdlow,Maha Barakat,Sherif R. Zaki +15 more
TL;DR: The histopathologic, immunohistochemical, and ultrastructural findings from the first autopsy performed on a fatal case of MERS-CoV in the world, which was related to a hospital outbreak in the United Arab Emirates in April 2014, provide critical insights into the pathogenesis of MSPV in humans.
Journal ArticleDOI
Serological assays for emerging coronaviruses: challenges and pitfalls.
TL;DR: The performance of different serological assays as well as the applicability of the two main applied antigens used during the SARS-CoV outbreak are summarized and a recommendation for a serological testing scheme is given and necessary improvements are outlined for a better preparedness.
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