Journal ArticleDOI
Cloning and sequence analysis of cDNA for mouse prolactin
Toshio Harigaya,Kazuhisa Nakayama,Hiroaki Ohkubo,Shigetada Nakanishi,Hisao Seo,Kazumas Hoshino +5 more
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TLDR
No sexual difference was revealed in murine prolactin mRNA and S1 nuclease mapping analysis using male and female pituitary RNAs indicates that mouse preprolactin is encoded by two mRNAs in both sexes.About:
This article is published in Biochimica et Biophysica Acta.The article was published on 1986-10-16. It has received 15 citations till now. The article focuses on the topics: Peptide sequence & cDNA library.read more
Citations
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The role of prolactin in fish osmoregulation: a review.
TL;DR: Much of the recent work has focused on the isolation and characterization of fish PRLs and their receptors, which have provided the necessary tools to obtain a better understanding of the evolution of PRL and its role in osmoregulation.
Journal ArticleDOI
A high-throughput screening of genes that encode proteins transported into the endoplasmic reticulum in mammalian cells
TL;DR: A new method based on the reconstitution of two split fragments of enhanced green fluorescent protein by protein splicing with DnaE from Synechocystis PCC6803 was developed, able to accurately identify cDNAs that encode proteins transported to the ER.
Journal ArticleDOI
Cloning of a turkey prolactin cDNA: expression of prolactin mRNA throughout the reproductive cycle of the domestic turkey (Meleagris gallopavo).
TL;DR: This study provides the first characterization of pituitary PRL mRNA and its comparison with plasma and pituitARY PRL levels during the avian reproductive cycle and appears to be related to the changes in PRL-releasing activity observed at each of the reproductive stages.
Journal ArticleDOI
Primary structure of chicken pituitary prolactin deduced from the cDNA sequence. Conserved and specific amino acid residues in the domains of the prolactins.
Masanori Watahiki,Minoru Tanaka,N Masuda,Kiyoko Sugisaki,Masanori Yamamoto,Minoru Yamakawa,Jun Nagai,Kunio Nakashima +7 more
TL;DR: The perform of chicken prolactin (PRL) deduced from the cDNA sequence contains a signal peptide of 30 amino acid residues followed by a mature PRL of 199 residues, suggesting that these residues are indispensable for specific binding of the PRLs to their receptors.
Journal ArticleDOI
Reproductive actions of prolactin mediated through short and long receptor isoforms
Y. Sangeeta Devi,Julia Halperin +1 more
TL;DR: The essential finding that both long and short receptor isoforms are crucial for ovarian functions and female fertility is described, and novel mechanisms of action for these receptors are highlighted.
References
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Detection of specific sequences among DNA fragments separated by gel electrophoresis.
TL;DR: This paper describes a method of transferring fragments of DNA from agarose gels to cellulose nitrate filters that can be hybridized to radioactive RNA and hybrids detected by radioautography or fluorography.
Journal ArticleDOI
Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
TL;DR: In this article, the rat pancreas RNA was used as a source for the purification of alpha-amylase messenger ribonucleic acid (RBA) using 2-mercaptoethanol.
Journal ArticleDOI
Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors
TL;DR: New Escherichia coli host strains have been constructed for the E. coli bacteriophage M13 and the high-copy-number pUC-plasmid cloning vectors and mutations introduced into these strains improve cloning of unmodified DNA and of repetitive sequences.
Book ChapterDOI
Sequencing end-labeled DNA with base-specific chemical cleavages.
Allan M. Maxam,Walter Gilbert +1 more
TL;DR: The chapter presents techniques for producing discrete DNA fragments, end-labeling DNA, segregating end- labeled fragments, extracting DNA from gels, and the protocols for partially cleaving it at specific bases using the chemical reactions.
Journal ArticleDOI
Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I
TL;DR: Labeled DNAs (and restriction endonuclease fragments derived from them) are useful probes for detecting rare homologous sequences by in situ hybridization and reassociation kinetic analysis.