Cloning, sequence, and expression of a human granulocyte/macrophage colony-stimulating factor.
Michael A. Cantrell,Dirk M. Anderson,Douglas P. Cerretti,Virginia L. Price,Kate N. Mckereghan,Robert J. Tushinski,Diane Y. Mochizuki,Åsmund Larsen,Kenneth H. Grabstein,Steven Gillis +9 more
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TLDR
One of these cDNA clones was shown to direct the synthesis of biologically active GM-CSF using a yeast expression system and appears to exist as a single-copy gene.Abstract:
Human granulocyte/macrophage colony-stimulating factor (GM-CSF) is a glycoprotein that is essential for the in vitro proliferation and differentiation of precursor cells into mature granulocytes and macrophages. In this report we have used a mouse GM-CSF cDNA clone to isolate human GM-CSF clones from libraries made from HUT-102 messenger RNA and mitogen-stimulated T-lymphocyte messenger RNA. The human cDNA clones contained a single open-reading frame encoding a protein of 144 amino acids with a predicted molecular mass of 16,293 daltons and showed 69% nucleotide homology and 54% amino acid homology to mouse GM-CSF. One of these cDNA clones was shown to direct the synthesis of biologically active GM-CSF using a yeast expression system. The gene for human GM-CSF appears to exist as a single-copy gene.read more
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A Short Polypeptide Marker Sequence Useful for Recombinant Protein Identification and Purification.
Thomas P. Hopp,K S Prickett,Virginia L. Price,Randell T. Libby,Carl J. March,Douglas P. Cerretti,David L. Urdal,Paul J. Conlon +7 more
TL;DR: A small hydrophilic peptide of eight amino acids was engineered onto the N-terminus of a variety of recombinant lymphokines for the purpose of aiding in their detection and purification from yeast supernatants or E. coli extracts.
Journal ArticleDOI
Establishment and characterization of a unique human cell line that proliferates dependently on GM-CSF, IL-3, or erythropoietin
Toshio Kitamura,Tsuyoshi Tange,Takashi Terasawa,Shigeru Chiba,Tomoaki Kuwaki,Kiyoshi Miyagawa,Yun-Feng Piao,Kohei Miyazono,Akio Urabe,Fumimaro Takaku +9 more
TL;DR: TF‐1 is a cell line of immature erythroid origin that requires GM‐CSF, IL‐3, or EPO for its growth and that has the ability to undergo differentiation into either more mature erystroid cells or into macrophage‐like cells.
Journal ArticleDOI
Granulocyte Colony-Stimulating Factor and Granulocyte-Macrophage Colony-Stimulating Factor
TL;DR: Four colony-stimulating factors influence the survival, proliferation, differentiation, and functional activation of myeloid hematopoietic cells: macrophage colony- Stimulating factor (M-CSF), granulocyte colony- stimulation factor (G- CSF, granulocytemacrophage colonies-stimulation factor (GM-CSf), and interleukin-3 (formerly known as multi-colony-stimulate factor).
PatentDOI
Activation of macrophage tumoricidal activity by granulocyte-macrophage colony stimulating factor
Dirk M. Anderson,Michael A. Cantrell,Douglas P. Cerretti,Conlon Iii Paul J,David J. Cosman,Kenneth H. Grabstein,Larsen Alf D,Virginia L. Price +7 more
TL;DR: In this paper, homogeneous granulocyte-macrophage colony stimulating factor for use in activating macrophages and monocyte precursors is prepared by recombinant DNA techniques.
Journal ArticleDOI
Effects of recombinant human granulocyte-macrophage colony-stimulating factor in patients with myelodysplastic syndromes.
Saroj Vadhan-Raj,Michael Keating,Michael Keating,Anne Lemaistre,Anne Lemaistre,Walter N. Hittelman,Walter N. Hittelman,Kenneth McCredie,Kenneth McCredie,Jose M. Trujillo,Jose M. Trujillo,Hal E. Broxmeyer,Hal E. Broxmeyer,Christopher S. Henney,Christopher S. Henney,Jordan U. Gutterman,Jordan U. Gutterman +16 more
TL;DR: Re recombinant human granulocyte--macrophage colony-stimulating factor (GM-CSF) is a potent stimulator of hematopoiesis in vivo and may produce hematologic improvement in the short term in patients with myelodysplastic syndrome.
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