Clostridium carboxidivorans sp. nov., a solvent-producing clostridium isolated from an agricultural settling lagoon, and reclassification of the acetogen Clostridium scatologenes strain SL1 as Clostridium drakei sp. nov.
Reads0
Chats0
TLDR
A novel solvent-producing, anaerobic clostridium, strain P7(T), was isolated from sediment from an agricultural settling lagoon after enrichment with CO as the substrate and analysis of the 16S rRNA gene sequence showed that it was closely related to Clostridial scatologenes ATCC 25775(T) (99.7% sequence similarity).Abstract:
A novel solvent-producing, anaerobic clostridium, strain P7T, was isolated from sediment from an agricultural settling lagoon after enrichment with CO as the substrate. The metabolism of this Gram-positive, motile, spore-forming rod was primarily acetogenic. Acetate, ethanol, butyrate and butanol were the end-products of metabolism. Strain P7T grew on CO, H2/CO2, glucose, galactose, fructose, xylose, mannose, cellobiose, trehalose, cellulose, starch, pectin, citrate, glycerol, ethanol, propanol, 2-propanol, butanol, glutamate, aspartate, alanine, histidine, asparagine, serine, betaine, choline and syringate as sole substrates. Growth was not supported by methanol, formate, d-arabinose, fucose, lactose, melibiose, amygdalin, gluconate, lactate, malate, arginine, glutamine or vanillate. Nitrate reduction, production of indole, gelatin hydrolysis and aesculin hydrolysis were not observed. Analysis of the 16S rRNA gene sequence of the isolate showed that it was closely related to Clostridium scatologenes ATCC 25775T (99·7 % sequence similarity) and clostridial strain SL1T (99·8 % sequence similarity). Strain SL1 had been classified as a strain of C. scatologenes. However, DNA–DNA reassociation analysis showed that both strain P7T and strain SL1 represented novel clostridial species. It is proposed that strain P7T (=ATCC BAA-624T=DSM 15243T) be classified as the type strain of Clostridium carboxidivorans sp. nov. and that strain SL1T (=ATCC BAA-623T=DSM 12750T) be reclassified as the type strain of Clostridium drakei sp. nov.read more
Citations
More filters
Journal ArticleDOI
Effect of salinity on C1-gas fermentation by Clostridium carboxidivorans producing acids and alcohols.
TL;DR: The possible inhibitory effect exerted by changes in salinity in the bioreactor was estimated in batch bottles assays, carried out with different salinities (media with different concentrations of sodium chloride) using C. carboxidivorans and CO as a carbon source.
Journal ArticleDOI
Electricity-enhanced anaerobic, non-photosynthetic mixotrophy by Clostridium carboxidivorans with increased carbon efficiency and alcohol production
Chi Wang Cheng,Yi-Min Shao,Weiming Li,Jiaxin Liu,Xiangzhi Liu,Yue Zhao,Xinqi Li,Shang-Tian Yang,Chuang Xue +8 more
TL;DR: In this article , the authors used a bio-electrochemical system (BES) to supply electrons to fix CO2 and shift the metabolite profiles towards more-reduced products in acetogenic bacteria Clostridium carboxidivorans.
Journal ArticleDOI
Mixotrophic co-utilization of glucose and carbon monoxide boosts ethanol and butanol productivity of continuous Clostridium carboxidivorans cultures.
TL;DR: In this paper , continuous cultivations of C.carboxidivorans to study heterotrophic and mixotrophic conversion of glucose and H2, CO2, and CO were established.
Journal ArticleDOI
Heat shocking of Clostridium strain P11 to promote sporulation and ethanol production.
TL;DR: In this paper, heat shocking of P11 cells prior to using them for producer gas fermentation enhanced ethanol production, while different methods have been employed to enhance product concentrations, heat shocking is a physical parameter that is less investigated.
References
More filters
Journal ArticleDOI
Colorimetric Method for Determination of Sugars and Related Substances
TL;DR: In this article, a method was developed to determine submicro amounts of sugars and related substances using a phenol-sulfuric acid reaction, which is useful for the determination of the composition of polysaccharides and their methyl derivatives.
Journal ArticleDOI
Confidence limits on phylogenies: an approach using the bootstrap.
TL;DR: The recently‐developed statistical method known as the “bootstrap” can be used to place confidence intervals on phylogenies and shows significant evidence for a group if it is defined by three or more characters.
Book
Bergey's Manual of Systematic Bacteriology
TL;DR: BCL3 and Sheehy cite Bergey's manual of determinative bacteriology of which systematic bacteriology, first edition, is an expansion.
Journal ArticleDOI
16S ribosomal DNA amplification for phylogenetic study.
TL;DR: A set of oligonucleotide primers capable of initiating enzymatic amplification (polymerase chain reaction) on a phylogenetically and taxonomically wide range of bacteria is described in this paper.