Journal ArticleDOI
Combining phage display and screening of cDNA expression libraries: a new approach for identifying the target antigen of an scFv preselected by phage display.
TLDR
Evidence is provided that the target antigen of a given scFv displayed on phages can be detected in an immobilized lambda phage cDNA expression library containing thousands of irrelevant clones.About:
This article is published in Journal of Molecular Biology.The article was published on 2000-08-25. It has received 22 citations till now. The article focuses on the topics: Phage display & Phagemid.read more
Citations
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Journal ArticleDOI
Protein quantification from complex protein mixtures using a proteomics methodology with single-cell resolution
TL;DR: With the linear amplification ability of T7 RNA polymerase, IDAT represents a significant improvement over immuno-PCR in terms of sensitivity and has the potential to provide a robotic platform for proteomics.
Patent
Methods and compositions for amplification of rna sequences
TL;DR: In this article, the authors proposed a method for isothermal amplification of RNA, which employs a composite primer, a second primer and strand displacement to generate multiple copies of DNA sequences complementary to an RNA sequence of interest.
Journal ArticleDOI
Receptor for the globular heads of C1q (gC1q-R, p33, hyaluronan-binding protein) is preferentially expressed by adenocarcinoma cells.
Daniel B. Rubinstein,Alexei Stortchevoi,Michael S. Boosalis,Raheela Ashfaq,Berhane Ghebrehiwet,Ellinor I.B. Peerschke,Fabien Calvo,Thierry Guillaume +7 more
TL;DR: The finding of tumor overexpression and the known multivalent binding of gC1q‐R to not only C1q itself but also a variety of circulating plasma proteins as well as its involvement in cell‐to‐cell interactions suggest that gC2qR may have a role in tumor metastases and potentially serve in molecule‐specific targeting of malignant cells.
Book ChapterDOI
Phage display for epitope determination: a paradigm for identifying receptor-ligand interactions
TL;DR: The techniques developed for mapping of antibody epitopes are applicable to probing the origins of autoimmune diseases and certain cancers by identifying "immunofootprints" of unknown initiating agents, and are directly applicable to examination of a wider range of receptor-ligand interactions.
Patent
Methods and compositions for transcription-based nucleic acid amplification
TL;DR: In this paper, isothermal exponential amplification of a target polynucleotide is described, which employs two transcription modules, the first module providing linear amplification resulting in RNA transcripts, and the second module providing for further cyclical amplification, resulting in more RNA transcripts.
References
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Journal ArticleDOI
Three different fibronectin mRNAs arise by alternative splicing within the coding region.
TL;DR: There are at least three different fibronectin mRNAs in rat liver which differ in coding potential and are probably all encoded by a single gene, according to the sequence data and S1 nuclease mapping.
Journal ArticleDOI
Antibody phage display technology and its applications.
TL;DR: A combination of the design and generation of millions to billions of different ligands, together with phage display for the isolation of binding ligands and with functional assays for identifying (and possibly selecting) bio-active ligands will provide a powerful tool for drug and target discovery well into the next decade.
Journal ArticleDOI
Characterization of human colon cancer antigens recognized by autologous antibodies
Matthew J. Scanlan,Yao-Tseng Chen,Yao-Tseng Chen,Barbara Williamson,Ali O. Gure,Elisabeth Stockert,John D. Gordan,Özlem Türeci,Ugur Sahin,Michael Pfreundschuh,Lloyd J. Old +10 more
TL;DR: The results on colon cancer illustrate both the complexity and the potential of the SEREX approach for analysis of the humoral immune response against human cancer.
Journal ArticleDOI
Strategies for selection of antibodies by phage display
TL;DR: Phage antibody-display is rapidly maturing into a very effective tool for antibody generation and successful strategies have also been developed to affinity mature these antibodies into the picomolar range if required.
Journal ArticleDOI
Isolation of a high-affinity stable single-chain Fv specific for mesothelin from DNA-immunized mice by phage display and construction of a recombinant immunotoxin with anti-tumor activity
TL;DR: It is shown that DNA immunization can be used to isolate and clone antibodies against epitopes present on human proteins in their native conformation and makes the immunotoxin a good candidate for development as a therapeutic agent.