James Eberwine

TL;DR: An open comprehensive reference map of the molecular state of cells in healthy human tissues would propel the systematic study of physiological states, developmental trajectories, regulatory circuitry and interactions of cells, and also provide a framework for understanding cellular dysregulation in human disease.

TL;DR: A method for producing amplified heterogeneous populations of RNA from limited quantities of cDNA and sequences for cyclophilin and guanine nucleotide-binding protein (G-protein) alpha subunits have been detected in aRNA derived from single cerebellar tissue sections.

TL;DR: Electrophysiological analysis coupled with molecular biology within the same cell will facilitate a better understanding of how changes at the molecular level are manifested in functional properties.

TL;DR: The combination of point-directed transfection and mRNATransfection is a new way of studying the function of genes and gene products in cells by using a precisely controlled laser-microcope system.

TL;DR: Examining a subset of these mRNAs and their encoded proteins in brain tissue from Fmr1 knockout mice, it is observed that some of these cargoes as well as the proteins they encode show discrete changes in abundance and/or differential subcellular distribution, consistent with spatially selective regulation of multiple biological pathways by FMRP.