Complex humoral immune response against a benign tumor: frequent antibody response against specific antigens as diagnostic targets.
Nicole Comtesse,Andrea Zippel,Sascha Walle,Dominik Monz,Christina Backes,Ulrike Fischer,Jens Mayer,Nicole Ludwig,Andreas Hildebrandt,Andreas Keller,Wolf-Ingo Steudel,Hans-Peter Lenhof,Eckart Meese +12 more
TLDR
A highly complex but specific humoral immune response against a benign tumor with a distinct serum reactivity pattern and a decline of complexity with malignancy is shown.Abstract:
There are numerous studies on the immune response against malignant human tumors. This study was aimed to address the complexity and specificity of humoral immune response against a benign human tumor. We assembled a panel of 62 meningioma-expressed antigens that show reactivity with serum antibodies of meningioma patients, including 41 previously uncharacterized antigens by screening of a fetal brain expression library. We tested the panel for reactivity with 48 sera, including sera of patients with common-type, atypical, and anaplastic meningioma, respectively. Meningioma sera detected an average of 14.6 antigens per serum and normal sera an average of 7.8 antigens per serum (P = 0.0001). We found a decline of seroreactivity with malignancy with a statistical significant difference between common-type and anaplastic meningioma (P < 0.05). We detected 17 antigens exclusively with patient sera, including 12 sera that were reactive against KIAA1344, 9 against natural killer tumor recognition (NKTR), and 7 against SRY (sex determining region Y)-box2 (SOX2). More than 80% of meningioma patients had antibodies against at least one of the antigens KIAA1344, SC65, SOX2, and C6orf153. Our results show a highly complex but specific humoral immune response against a benign tumor with a distinct serum reactivity pattern and a decline of complexity with malignancy. The frequent antibody response against specific antigens offers new diagnostic and therapeutic targets for meningioma. We developed a statistical learning method to differentiate sera of meningioma patients from sera of healthy donors.read more
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Sox2 expression in breast tumours and activation in breast cancer stem cells.
Olatz Leis,Arrate Eguiara,Erika López-Arribillaga,M J Alberdi,Susana Hernández-García,K Elorriaga,Atanasio Pandiella,R Rezola,Angel G. Martin +8 more
TL;DR: It is suggested that reactivation of Sox2 represents an early step in breast tumour initiation, explaining tumour heterogeneity by placing the tumour-initiating event in any cell along the axis of mammary differentiation.
Journal ArticleDOI
The Molecular Mechanism Governing the Oncogenic Potential of SOX2 in Breast Cancer
Yupeng Chen,Lei Shi,Lirong Zhang,Ruifang Li,Jing Liang,Wenhua Yu,Luyang Sun,Xiaohan Yang,Yan Wang,Yu Zhang,Yongfeng Shang +10 more
TL;DR: Using cell culture experiments, tissue analysis, molecular profiling, and animal studies, it is reported here that SOX2 promotes cell proliferation and tumorigenesis by facilitating the G1/S transition and through its transcription regulation of the CCND1 gene in breast cancer cells.
Journal ArticleDOI
Evidence That SOX2 Overexpression Is Oncogenic in the Lung
Yun Lu,Christopher R. Futtner,Jason R. Rock,Xia Xu,Walter Whitworth,Brigid L.M. Hogan,Mark W. Onaitis +6 more
TL;DR: These findings demonstrate that Sox2 overexpression both induces a proximal phenotype in the distal airways/alveoli and leads to cancer.
Journal ArticleDOI
Expression of the embryonic stem cell marker SOX2 in early-stage breast carcinoma
Claudia Lengerke,Tanja Fehm,Ralf Kurth,Hans Neubauer,Veit Scheble,Friederike Müller,Friederike Schneider,Karen Petersen,Diethelm Wallwiener,Lothar Kanz,Falko Fend,Sven Perner,Petra M. Bareiss,Annette Staebler +13 more
TL;DR: It is shown that the embryonic stem cell factor SOX2 is expressed in a variety of early stage postmenopausal breast carcinomas and metastatic lymph nodes and high expression may promote metastatic potential.
Journal ArticleDOI
Frequent and specific immunity to the embryonal stem cell–associated antigen SOX2 in patients with monoclonal gammopathy
Radek Spisek,Anjli Kukreja,Lin-Chi Chen M.D.,Phillip Matthews,Amitabha Mazumder,David H. Vesole,Sundar Jagannath,Henry Zebroski,Andrew J. G. Simpson,Gerd Ritter,Brian G.M. Durie,John Crowley,John D. Shaughnessy,Matthew J. Scanlan,Ali O. Gure,Ali O. Gure,Bart Barlogie,Madhav V. Dhodapkar +17 more
TL;DR: It is shown that antigenic targets of spontaneous immunity in MGUS differ from MM, and detection of anti-SOX2 T cells predicts favorable clinical outcome in patients with asymptomatic plasmaproliferative disorders.
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