Construction of a full-length cDNA library and analysis of expressed sequence tags in white jute (Corchorus capsularis L.)
Aifen Tao,Xiaozhen Li,Jianmin Qi,Pingping Fang,Lihui Lin,Jiantang Xu,Liwu Zhang,Wu Jianmei,Lin Peiqing +8 more
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TLDR
A complementary DNA library of white jute was constructed and expressed sequence tags (ESTs) were characterized, indicating that unigenes of C. capsularis have higher homology to Populus trichocarpa, Ricinus communis and Corchorus olitorius.Abstract:
White jute ( Corchorus capsularis L.) is recognized as an important industrial raw material fibre crop owing to its elite characters. However, little information is known about its molecular basis and genomics. In this study, a complementary DNA library of white jute was constructed and expressed sequence tags (ESTs) were characterized. The titers of original and amplified libraries were 2.32 × 10 7 and 1.07 × 10 9 pfu/mL, respectively. The recombinant frequency was 98.3% in the library. Most of the sequences ranged from 500 to 1500 bp with an average length of 750 bp. Results show 203 (73%) ESTs exhibited significant similarity with known or putative functional nucleotide sequences in the GenBank databases. Cluster analysis allowed the identification of 61 unique sequences. These genes were classified into six types by Gene Ontology (GO) annotation. The results also indicated that unigenes of C. capsularis have higher homology to Populus trichocarpa , Ricinus communis and Corchorus olitorius . This report will provide a valuable resource for the further investigations in the gene cloning, transcription or expression for white jute. Key words: White jute, Corchorus capsularis, cDNA library, construction, ESTs, analysis.read more
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Book ChapterDOI
Jute Genomics : Emerging Resources and Tools for Molecular Breeding
TL;DR: The present status of jute genomics is discussed, with a historical perspective on DNA markers development and utilization, and the potentials of genomic selection to accelerate the rate of genetic gain in selection for bast fibre quality traits and mutagenesis-based reverse genetic approach for developing low-lignin jute are discussed.
Book ChapterDOI
Fiber crop, jute improvement by using genomics and genetic engineering
TL;DR: Better understanding of many aspects of jute genome size, chromosome structure, genome sequencing, tissue culture regeneration, stable and efficient genetic transformation system development, and the application of genetic engineering to develop insect-resistant, fungus- resistant, and herbicide-tolerant varieties are studied.
References
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