Cost-effectiveness of blood agar for isolation of mycobacteria.
Michel Drancourt,Didier Raoult +1 more
Reads0
Chats0
TLDR
Blood agar should be regarded as a first-line medium for culturing Mycobacterium species, it saves time, is cost-effective, is more sensitive than, and at least as rapid as the automated method, and for resource-limited countries in which the prevalence of tuberculosis is high.Abstract:
BackgroundMycobacterium species are grown using specific media that increase laboratory cost, thus hampering their diffusion in resource-limited countries. Preliminary data suggested that versatile blood agar may be also used for mycobacterial culture.MethodologyWe examined the growth of 41 different Mycobacterium species on 5% blood agar. Over a 24-month period we analysed isolation of mycobacteria after parallel inoculation of clinical specimens into both a reference automated system (BACTEC 9000 MB broth) and 5% blood agar slant tubes, after NaOH decontamination, and compared the cost of performing 1,000 analyses using these two techniques.ConclusionsMycobacterium reference species cultured on blood agar, with the exception of Mycobacterium ulcerans. Inoculation of 1,634 specimens yielded 95 Mycobacterium isolates. Blood agar performed significantly more efficiently than BACTEC 9000 MB broth (94 vs 88 isolates, P = 0.03). Decontamination of Candida albicans in 5 specimens by addition of amphotericin B in blood agar yielded one more M. tuberculosis isolate that could not be isolated in BACTEC broth. Uneven distribution of time to culture positivity for M. tuberculosis had a median (range) of 19±5 days using blood agar and 26±6 days using BACTEC 9000 MB broth. Cost for 1,000 analyses in France was estimated to be of 1,913 euros using the blood agar method and 8,990 euros using the BACTEC 9000 MB method. Blood agar should be regarded as a first-line medium for culturing Mycobacterium species. It saves time, is cost-effective, is more sensitive than, and at least as rapid as the automated method. This is of particular importance for resource-limited countries in which the prevalence of tuberculosis is high.read more
Citations
More filters
Journal ArticleDOI
Current and Past Strategies for Bacterial Culture in Clinical Microbiology
Jean-Christophe Lagier,Sophie Edouard,Isabelle Pagnier,Oleg Mediannikov,Michel Drancourt,Didier Raoult +5 more
TL;DR: The development of axenic media for the culture of Treponema pallidum or Mycobacterium leprae remains an important challenge that the patience and innovations of cultivators will enable them to overcome.
Journal ArticleDOI
Modern clinical microbiology: new challenges and solutions
Pierre-Edouard Fournier,Michel Drancourt,Philippe Colson,Jean-Marc Rolain,Bernard La Scola,Didier Raoult +5 more
TL;DR: New technologies, including mass spectrometry for colony identification, real-time genomics for isolate characterization, and versatile and permissive culture systems can improve the workflow and output of clinical microbiology laboratories and, by identifying and characterizing microbial pathogens, provide significant input to scientific discovery.
Journal ArticleDOI
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry identification of mycobacteria in routine clinical practice.
TL;DR: The data indicate that MALDI-TOF MS can be used as a first-line method for the routine identification of heat-inactivated mycobacteria and is an attractive method for implementation in clinical microbiology laboratories in both developed and developing countries.
Journal ArticleDOI
Steps towards the discovery of Mycobacterium tuberculosis by Robert Koch, 1882
E. Cambau,Michel Drancourt +1 more
TL;DR: It is thanks to R. Koch that one of the most lethal diseases in human history could be diagnosed, could be treated and cured after the discovery of streptomycin 65 years later, and could be efficiently prevented by isolation of cases.
Journal ArticleDOI
Repertoire of intensive care unit pneumonia microbiota
Sabri Bousbia,Laurent Papazian,Pierre Saux,Jean Marie Forel,Jean-Pierre Auffray,Claude Martin,Didier Raoult,Bernard La Scola +7 more
TL;DR: Some pathogens considered to be typical for ICU pneumonia such as Pseudomonas aeruginosa and Streptococcus species can be detected as commonly in controls as in pneumonia patients which strikingly highlights the existence of a core pulmonary microbiota.
References
More filters
Journal ArticleDOI
rpoB-Based Identification of Nonpigmented and Late-Pigmenting Rapidly Growing Mycobacteria
TL;DR: It is concluded that molecular identification by analysis of the 723-bp rpoB sequence is a rapid and accurate tool for identification of RGM.
Journal ArticleDOI
Microscopic-Observation Drug-Susceptibility Assay for the Diagnosis of TB
David Moore,Carlton A. Evans,Robert H. Gilman,Luz Caviedes,Jorge Coronel,Aldo Vivar,Eduardo García Sánchez,Yvette Piñedo,Juan Carlos Saravia,Cayo Salazar,Richard A. Oberhelman,Maria Graciela Hollm-Delgado,Doris LaChira,A. Roderick Escombe,A. Roderick Escombe,Jon S. Friedland +15 more
TL;DR: A single MODS culture of a sputum sample offers more rapid and sensitive detection of tuberculosis and multidrug-resistant tuberculosis than the existing gold-standard methods used.
Journal ArticleDOI
Sputum digestion and decontamination with N-acetyl-L-cysteine-sodium hydroxide for culture of mycobacteria.
Journal ArticleDOI
Buruli ulcer: emerging from obscurity
TL;DR: Considerable progress has been made in understanding the immune response to M ulcerans and there have been major advances in management of the disease with the introduction of rational antibiotic therapy.
Journal ArticleDOI
Lymph node biopsy specimens and diagnosis of cat-scratch disease.
Jean-Marc Rolain,Hubert Lepidi,Michel Zanaret,Jean-Michel Triglia,Gérard Michel,Pascal-Alexandre Thomas,Michèle Texereau,Andreas Stein,Anette Romaru,François Eb,Didier Raoult +10 more
TL;DR: Histologic analysis of lymph node biopsy specimens may verify diagnosis of this disease.