Covalently Adaptable Elastin-Like Protein-Hyaluronic Acid (ELP-HA) Hybrid Hydrogels with Secondary Thermoresponsive Crosslinking for Injectable Stem Cell Delivery
TLDR
Improved structural integrity enables cell culture for three weeks postinjection, and encapsulated cells maintain their ability to differentiate into multiple lineages, including chondrogenic, adipogenic, and osteogenic cell types.Abstract:
Shear-thinning, self-healing hydrogels are promising vehicles for therapeutic cargo delivery due to their ability to be injected using minimally invasive surgical procedures An injectable hydrogel using a novel combination of dynamic covalent crosslinking with thermoresponsive engineered proteins is presented Ex situ at room temperature, rapid gelation occurs through dynamic covalent hydrazone bonds by simply mixing two components: hydrazine-modified elastin-like protein (ELP) and aldehyde-modified hyaluronic acid This hydrogel provides significant mechanical protection to encapsulated human mesenchymal stem cells during syringe needle injection and rapidly recovers after injection to retain the cells homogeneously within a 3D environment In situ, the ELP undergoes a thermal phase transition, as confirmed by coherent anti-Stokes Raman scattering microscopy observation of dense ELP thermal aggregates The formation of the secondary network reinforces the hydrogel and results in a tenfold slower erosion rate compared to a control hydrogel without secondary thermal crosslinking This improved structural integrity enables cell culture for three weeks postinjection, and encapsulated cells maintain their ability to differentiate into multiple lineages, including chondrogenic, adipogenic, and osteogenic cell types Together, these data demonstrate the promising potential of ELP-HA hydrogels for injectable stem cell transplantation and tissue regenerationread more
Citations
More filters
Journal ArticleDOI
3D printing of hydrogels: Rational design strategies and emerging biomedical applications
TL;DR: A review of hydrogel-based biomaterial inks and bioinks for 3D printing can be found in this paper, where the authors provide a comprehensive overview and discussion of the tailorability of material, mechanical, physical, chemical and biological properties.
Journal ArticleDOI
Highly Efficient Self-Healable and Dual Responsive Cellulose-Based Hydrogels for Controlled Release and 3D Cell Culture
Xuefeng Yang,Guoqiang Liu,Liao Peng,Jinhua Guo,Lei Tao,Jinying Yuan,Chunyu Chang,Yen Wei,Lina Zhang,Lina Zhang +9 more
TL;DR: Benefitting from the excellent biocompatibility and the reversibly cross‐linked networks, the cellulose‐based self‐healing hydrogels can function as suitable 3D culture scaffolds for L929 cells, leading to the encapsulated cells maintaining a high viability and proliferative capacity.
Journal ArticleDOI
Toward Stimuli-Responsive Dynamic Thermosets through Continuous Development and Improvements in Covalent Adaptable Networks (CANs)
Maciej Podgórski,Benjamin D. Fairbanks,Bruce E. Kirkpatrick,Matthew K. McBride,Alina M. Martinez,Adam Dobson,Nicholas J. Bongiardina,Christopher N. Bowman +7 more
TL;DR: Recent developments in covalent adaptable networks are discussed here with an emphasis on the most effective dynamic chemistries that render these materials to be stimuli responsive, enabling features that make CANs more broadly applicable.
Journal ArticleDOI
Methods To Assess Shear-Thinning Hydrogels for Application As Injectable Biomaterials
TL;DR: Methods for delivery of hydrogels to mouse, rat, and pig hearts in models of myocardial infarction are discussed, and methods of tissue postprocessing for hydrogel preservation are compared.
Journal ArticleDOI
Hydrogels Based on Schiff Base Linkages for Biomedical Applications.
TL;DR: The design and preparation of hydrogels based on various types of Schiff base linkages are summarized, as well as the biomedical applications ofhydrogels in drug delivery, tissue regeneration, wound healing, tissue adhesives, bioprinting, and biosensors are summarized.
References
More filters
Journal ArticleDOI
Hydrogels for tissue engineering: scaffold design variables and applications.
Jeanie L. Drury,David J. Mooney +1 more
TL;DR: Hydrogels are an appealing scaffold material because they are structurally similar to the extracellular matrix of many tissues, can often be processed under relatively mild conditions, and may be delivered in a minimally invasive manner.
Journal ArticleDOI
Synthetic biomaterials as instructive extracellular microenvironments for morphogenesis in tissue engineering
TL;DR: Although modern synthetic biomaterials represent oversimplified mimics of natural ECMs lacking the essential natural temporal and spatial complexity, a growing symbiosis of materials engineering and cell biology may ultimately result in synthetic materials that contain the necessary signals to recapitulate developmental processes in tissue- and organ-specific differentiation and morphogenesis.
Journal ArticleDOI
Hydrogels in Biology and Medicine: From Molecular Principles to Bionanotechnology†
TL;DR: This work highlights recent developments in engineering uncrosslinked and crosslinked hydrophilic polymers for biomedical and biological applications and shows how such systems' intelligent behavior can be used in sensors, microarrays, and imaging.
Journal ArticleDOI
Hydrogels in regenerative medicine
Brandon V. Slaughter,Shahana S. Khurshid,Omar Z. Fisher,Ali Khademhosseini,Nicholas A. Peppas +4 more
TL;DR: The properties of hydrogels that are important for tissue engineering applications and the inherent material design constraints and challenges are discussed.
Journal ArticleDOI
Hydrogels as Extracellular Matrix Mimics for 3D Cell Culture
TL;DR: The use of both synthetic and natural hydrogels as scaffolds for three-dimensional cell culture as well as synthetic hydrogel hybrids that incorporate sophisticated biochemical and mechanical cues as mimics of the native extracellular matrix are discussed.
Related Papers (5)
Adaptable Hydrogel Networks with Reversible Linkages for Tissue Engineering
Huiyuan Wang,Sarah C. Heilshorn +1 more
Hydrogels with tunable stress relaxation regulate stem cell fate and activity
Ovijit Chaudhuri,Ovijit Chaudhuri,Ovijit Chaudhuri,Luo Gu,Luo Gu,Darinka D. Klumpers,Darinka D. Klumpers,Darinka D. Klumpers,Max Darnell,Max Darnell,Sidi A. Bencherif,Sidi A. Bencherif,James C. Weaver,Nathaniel Huebsch,Hong-pyo Lee,Evi Lippens,Evi Lippens,Georg N. Duda,David J. Mooney,David J. Mooney +19 more