Deletion of the Igκ Light Chain Intronic Enhancer/Matrix Attachment Region Impairs but Does Not Abolish VκJκ Rearrangement
TLDR
Little V kappa J kappa rearrangement could be detected in B cells derived from E kappa NI ES cells, demonstrating that an inserted PGK-neo(r) gene dominantly suppresses V k Kappa J kappas rearrangements.About:
This article is published in Immunity.The article was published on 1996-04-01 and is currently open access. It has received 176 citations till now. The article focuses on the topics: Gene rearrangement & Kappa.read more
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Targeted disruption of ATM leads to growth retardation, chromosomal fragmentation during meiosis, immune defects, and thymic lymphoma.
TL;DR: Findings indicate that the ATM gene product plays an essential role in a diverse group of cellular processes, including meiosis, the normal growth of somatic tissues, immune development, and tumor suppression.
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NKG2D-Deficient Mice Are Defective in Tumor Surveillance in Models of Spontaneous Malignancy
Nadia Guerra,Ying Xim Tan,Nathalie T. Joncker,Augustine Choy,Fermin Gallardo,Na Xiong,Sue E. Knoblaugh,Dragana Cado,Norman R. Greenberg,David H. Raulet +9 more
TL;DR: Evidence is provided that NKG2D was not necessary for NK cell development but was critical for immunosurveillance of epithelial and lymphoid malignancies in two transgenic models of de novo tumorigenesis, providing important genetic evidence for surveillance of primary tumors by an NK receptor.
Journal ArticleDOI
Lhx2, a LIM homeobox gene, is required for eye, forebrain, and definitive erythrocyte development.
Forbes D. Porter,John Drago,Yang Xu,Surindar S. Cheema,Christopher A. Wassif,Sing-Ping Huang,Eric Lee,Alexander Grinberg,Jim S. Massalas,David M. Bodine,Frederick W. Alt,Heiner Westphal +11 more
TL;DR: The function of Lhx2, a LIM homeobox gene expressed in developing B-cells, forebrain and neural retina, is investigated by analyzing embryos deficient in functional Lx2 protein, finding it necessary for normal development of the eye, cerebral cortex, and efficient definitive erythropoiesis.
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Dual roles of ATM in the cellular response to radiation and in cell growth control.
Yang Xu,David Baltimore +1 more
TL;DR: The gene mutated in ataxia-telangiectasia (AT) patients, denoted ATM, encodes a putative protein or lipid kinase that is critical both for cellular responses to ionizing radiation and for normal cell-cycle progression.
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Abnormal development of secondary lymphoid tissues in lymphotoxin beta-deficient mice.
Alimzhanov Mb,Dmitry V. Kuprash,Marie H. Kosco-Vilbois,Arne Luz,Regina L. Turetskaya,Alexander Tarakhovsky,Klaus Rajewsky,Sergei A. Nedospasov,Klaus Pfeffer +8 more
TL;DR: Results indicate that LTalpha can signal independently from LTbeta in the formation of PNA-positive foci in the spleen, and especially in the development of mesenteric and cervical LN.
References
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Targeted mutation of the DNA methyltransferase gene results in embryonic lethality.
TL;DR: Results indicate that while a 3-fold reduction in levels of genomic m5C has no detectable effect on the viability or proliferation of ES cells in culture, a similar reduction of DNA methylation in embryos causes abnormal development and embryonic lethality.
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Somatic generation of antibody diversity
TL;DR: In the genome of a germ-line cell, the genetic information for an immunoglobulin polypeptide chain is contained in multiple gene segments scattered along a chromosome which are assembled by recombination which leads to the formation of a complete gene.
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Multiple nuclear factors interact with the immunoglobulin enhancer sequences.
Ranjan Sen,David Baltimore +1 more
TL;DR: In this paper, an electrophoretic mobility shift assay with end-labeled DNA fragments was used to characterize proteins that bind to the immunoglobulin (Ig) heavy chain and the kappa light chain enhancers.
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Inducible gene targeting in mice
TL;DR: A method of gene targeting that allows the inducible inactivation of a target gene in mice is presented, which uses an interferon-responsive promoter to control the expression of Cre recombinase.
Journal Article
A New Mouse Myeloma Cell Line that Has Lost Immunoglobulin Expression but Permits the Construction of Antibody-Secreting Hybrid Cell Lines
TL;DR: A subclone of the mouse myeloma cell line P3-X63-Ag8 that does not express immunoglobulin heavy or light chains is isolated and can be used for efficient fusion with antibody-forming cells to obtain hybrid cell lines producing pure monoclonal antibodies.
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V(D)J recombination in B cells is impaired but not blocked by targeted deletion of the immunoglobulin heavy chain intron enhancer.
Matthias Serwe,Fred Sablitzky +1 more