Detection of Lactobacillus, Pediococcus, Leuconostoc, and Weissella Species in Human Feces by Using Group-Specific PCR Primers and Denaturing Gradient Gel Electrophoresis
TLDR
Denaturing gradient gel electrophoresis (DGGE) of DNA fragments generated by PCR with 16S ribosomal DNA-targeted group-specific primers was used to detect lactic acid bacteria (LAB) of the genera lactobacillus, Pediococcus, Leuconostoc, andWeissella in human feces.Abstract:
Denaturing gradient gel electrophoresis (DGGE) of DNA fragments generated by PCR with 16S ribosomal DNA-targeted group-specific primers was used to detect lactic acid bacteria (LAB) of the genera Lactobacillus, Pediococcus, Leuconostoc, and Weissella in human feces. Analysis of fecal samples of four subjects revealed individual profiles of DNA fragments originating not only from species that have been described as intestinal inhabitants but also from characteristically food-associated bacteria such as Lactobacillus sakei, Lactobacillus curvatus, Leuconostoc mesenteroides, and Pediococcus pentosaceus. Comparison of PCR-DGGE results with those of bacteriological culture showed that the food-associated species could not be cultured from the fecal samples by plating on Rogosa agar. On the other hand, all of the LAB species cultured from feces were detected in the DGGE profile. We also detected changes in the types of LAB present in human feces during consumption of a milk product containing the probiotic strain Lactobacillus rhamnosus DR20. The analysis of fecal samples from two subjects taken before, during, and after administration of the probiotic revealed that L. rhamnosus was detectable by PCR-DGGE during the test period in the feces of both subjects, whereas it was detectable by culture in only one of the subjects.read more
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Gut Microbiota in Human Adults with Type 2 Diabetes Differs from Non-Diabetic Adults
Nadja Larsen,Finn K. Vogensen,Frans van den Berg,Dennis Sandris Nielsen,Anne Sofie Andreasen,Bente Klarlund Pedersen,Waleed Abu Al-Soud,Søren J. Sørensen,Lars Hestbjerg Hansen,Mogens Jakobsen +9 more
TL;DR: The results of this study indicate that type 2 diabetes in humans is associated with compositional changes in intestinal microbiota and the level of glucose tolerance should be considered when linking microbiota with metabolic diseases such as obesity and developing strategies to control metabolic diseases by modifying the gut microbiota.
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Reduction in diversity of the colonic mucosa associated bacterial microflora in patients with active inflammatory bowel disease
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Individuality in gut microbiota composition is a complex polygenic trait shaped by multiple environmental and host genetic factors
Andrew K. Benson,Scott A. Kelly,Ryan Legge,Fangrui Ma,Soo Jen Low,Jaehyoung Kim,Min Zhang,Phaik Lyn Oh,Derrick L. Nehrenberg,Kunjie Hua,Stephen D. Kachman,Etsuko N. Moriyama,Jens Walter,Daniel A. Peterson,Daniel Pomp +14 more
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Development of an extensive set of 16S rDNA‐targeted primers for quantification of pathogenic and indigenous bacteria in faecal samples by real‐time PCR
TL;DR: The design and optimization of an extensive set of 16S rDNA‐targeted species‐ and group‐specific primers for more accurate quantification of bacteria from faecal samples with real‐time PCR are reported.
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