Detection of SARS-CoV-2 in a dog with hemorrhagic diarrhea
Miguel Padilla-Blanco,Santiago Vega,Luis Enjuanes,Alfonso Morey,Teresa Lorenzo,Clara Marin,Carmen Ivorra,Elisa Maiques,Vicente Rubio,Consuelo Rubio-Guerri +9 more
TLDR
In this article , the authors detected SARS-CoV-2 in a dog from the North African coastal Spanish city of Ceuta presenting hemorrhagic diarrhea, a disease also reported earlier on in an infected dog from USA.Abstract:
Abstract Background Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of COVID-19, has infected several animal species, including dogs, presumably via human-to-animal transmission. Most infected dogs reported were asymptomatic, with low viral loads. However, in this case we detected SARS-CoV-2 in a dog from the North African coastal Spanish city of Ceuta presenting hemorrhagic diarrhea, a disease also reported earlier on in an infected dog from the USA. Case presentation In early January 2021, a West Highland Terrier pet dog from Ceuta (Spain) presented hemorrhagic diarrhea with negative tests for candidate microbial pathogens. Since the animal was in a household whose members suffered SARS-CoV-2 in December 2020, dog feces were analyzed for SARS-CoV-2, proving positive in a two-tube RT-PCR test, with confirmation by sequencing a 399-nucleotide region of the spike ( S ) gene. Furthermore, next-generation sequencing (NGS) covered > 90% SARS-CoV-2 genome sequence, allowing to classify it as variant B.1.177. Remarkably, the sequence revealed the Ile402Val substitution in the spike protein (S), of potential concern because it mapped in the receptor binding domain (RBD) that mediates virus interaction with the cell. NGS reads mapping to bacterial genomes showed that the dog fecal microbiome fitted best the characteristic microbiome of dog’s acute hemorrhagic diarrhea. Conclusion Our findings exemplify dog infection stemming from the human SARS-CoV-2 pandemic, providing nearly complete-genome sequencing of the virus, which is recognized as belonging to the B.1.177 variant, adding knowledge on variant circulation in a geographic region and period for which there was little viral variant characterization. A single amino acid substitution found in the S protein that could have been of concern is excluded to belong to this category given its rarity and intrinsic nature. The dog’s pathology suggests that SARS-CoV-2 could affect the gastrointestinal tract of the dog. read more
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SARS-CoV-2 in Namibian Dogs
Umberto Molini,L. M. Coetzee,Tanya Engelbrecht,Lourens de Villiers,Mari de Villiers,Iolanda Mangone,Valentina Curini,Siegfried Khaiseb,Massimo Ancora,Cesare Cammà,Alessio Lorusso,Giovanni Franzo +11 more
TL;DR: In this paper , an extensive molecular biology survey was performed from March 2022 to September 2022 on Namibian dogs residing in urban and rural areas, showing a low but not negligible SARS-CoV-2 prevalence (1%; 95CI: 0.33-2.32%) of 5 out of 500.
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Serological screening of SARS-CoV-2 infection in companion animals of Buenos Aires suburbs
Nancy Patricia Cardoso,Carla L. G. Rivero,Mariángeles Castillo,Florencia Celeste Mansilla,Florencia Pastorino,Gustavo Martínez,Leticia V. Bentancor,Alejandra Victoria Capozzo +7 more
TL;DR: In this article , a multi-species indirect ELISA was developed to measure antibodies reactive to the SARS-CoV-2 receptor-binding domain (RBD) from several vertebrates constituting the class Mammalia, making it a valuable tool for field serosurveillance.
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Interactions between Humans and Dogs during the COVID-19 Pandemic: Recent Updates and Future Perspectives
TL;DR: In this paper , the authors focused on the human-canine interface of COVID-19 and the positives and negatives of this interface and found that dogs can get COVID19 from their owners but are asymptomatic when infected, but it is unclear if it affects the health of dogs.
Journal ArticleDOI
A SARS-CoV-2 full genome sequence of the B.1.1 lineage sheds light on viral evolution in Sicily in late 2020
Miguel Padilla-Blanco,Francesca Gucciardi,Vicente Rubio,Antonio Lastra,Teresa Lorenzo,Bea Ballester,Andrea González-Pastor,Veronica Veses,Giusi Macaluso,Chirag C. Sheth,Marina Pascual-Ortiz,Elisa Maiques,Consuelo Rubio-Guerri,Giuseppa Purpari,Annalisa Guercio +14 more
TL;DR: In this article , the authors characterized the occurrence in Sicily of viral lineages in the second pandemic wave (September to December 2020) and revealed wide prevalence of the then widespread through Europe B.1.177 variant, although some viral samples could not be classified with limited Sanger sequencing tools used.
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