Disturbed peripheral B lymphocyte homeostasis in systemic lupus erythematosus.
Marcus Odendahl,A.M. Jacobi,Arne Hansen,Eugen Feist,Falk Hiepe,Gerd R Burmester,Peter E. Lipsky,Andreas Radbruch,Thomas Dörner +8 more
TLDR
There are profound abnormalities in the various B cell compartments in SLE that respond differently to immunosuppressive therapy, and CD27high plasma cells showed a similar degree of somatic hypermutation, but preferentially employed VH4 family members.Abstract:
In patients with active systemic lupus erythematosus (SLE), a marked B lymphocytopenia was identified that affected CD19(+)/CD27(-) naive B cells more than CD19(+)/CD27(+) memory B cells, leading to a relative predominance of CD27-expressing peripheral B cells CD27(high)/CD38(+)/CD19(dim)/surface Ig(low)/CD20(-)/CD138(+) plasma cells were found at high frequencies in active but not inactive SLE patients Upon immunosuppressive therapy, CD27(high) plasma cells and naive CD27(-) B cells were markedly decreased in the peripheral blood Mutational analysis of V gene rearrangements of individual B cells confirmed that CD27(+) B cells coexpressing IgD were memory B cells preferentially using V(H)3 family members with multiple somatic mutations CD27(high) plasma cells showed a similar degree of somatic hypermutation, but preferentially employed V(H)4 family members These results indicate that there are profound abnormalities in the various B cell compartments in SLE that respond differently to immunosuppressive therapyread more
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Maintenance of Serological Memory by Polyclonal Activation of Human Memory B Cells
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Induction of Dendritic Cell Differentiation by IFN-α in Systemic Lupus Erythematosus
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TL;DR: Studies of the biology of plasma cells reveal a mechanism of intriguing simplicity and elegance that focuses memory provided by plasma cells on recently encountered pathogens while minimizing the 'fading' of memory for pathogens encountered in the distant past.
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References
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Human Immunoglobulin (Ig)M+IgD+ Peripheral Blood B Cells Expressing the CD27 Cell Surface Antigen Carry Somatically Mutated Variable Region Genes: CD27 as a General Marker for Somatically Mutated (Memory) B Cells
TL;DR: It is reported here that human IgM+IgD+ peripheral blood (PB) B cells expressing the CD27 cell surface antigen carry mutated V genes, in contrast to CD27-negative IgM-only tonsillar germinal center and plasma cells, which may represent a general marker for memory B cells in humans.
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Cellular Origin of Human B-Cell Lymphomas
TL;DR: The origin of human lymphomas has been studied by various approaches, including histology and immunophenotyping, but sequence analysis of the variable-region genes of B-cell lymphomas offered a molecular approach to studying the origin of the tumors.
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Analysis of somatic mutation in five B cell subsets of human tonsil.
TL;DR: It is found that the somatic mutation machinery is activated only after B cells reach the germinal center and become centroblasts (Bm3), and the analysis of Ig variable region transcripts from the different subpopulations confirms that the pathway of B cell differentiation from virgin B cell throughout the gerMinal center up to the memory compartment can be traced with phenotypic markers.
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Identification of Functional Human Splenic Memory B Cells by Expression of CD148 and CD27
TL;DR: Examining the expression of the receptor-type protein tyrosine phosphatase CD148 on human B cells identifies CD148 and CD27 as markers which positively identify memory B cells present in human spleen.