Journal ArticleDOI
E. coli RNA polymerase interacts homologously with two different promoters
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TLDR
The disposition of the contacts in space suggests a model for the pathway along which the RNA polymerase binds to promoters, as shown on three-dimensional models.About:
This article is published in Cell.The article was published on 1980-06-01. It has received 827 citations till now. The article focuses on the topics: Pribnow box & RNA polymerase I.read more
Citations
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Journal ArticleDOI
Compilation and analysis of Escherichia coli promoter DNA sequences.
TL;DR: A consensus promoter sequence based on homologies among 112 well-defined promoters was determined that was in substantial agreement with previous compilations.
Journal ArticleDOI
Complete nucleotide sequence of bacteriophage T7 DNA and the locations of T7 genetic elements
TL;DR: The complete nucleotide sequence of bacteriophage T7 DNA, 39,936 base-pairs, has been determined and the amino acid sequences and compositions predicted for all of the T7 proteins (except the proteins produced by frameshifting) are given.
Journal ArticleDOI
The tac promoter: a functional hybrid derived from the trp and lac promoters
TL;DR: Two hybrid promoters that are functional in Escherichia coli have been constructed and are useful for the controlled expression of foreign genes at high levels in E. coli.
Journal ArticleDOI
Weight matrix descriptions of four eukaryotic RNA polymerase II promoter elements derived from 502 unrelated promoter sequences
TL;DR: Optimized weight matrices defining four major eukaryotic promoter elements, the TATA-box, cap signal, CCAAT-, and GC-box are presented; they were derived by comparative sequence analysis of 502 unrelated RNA polymerase II promoter regions by a novel algorithm that is generally applicable to sequence motifs positionally correlated with a biologically defined position in the sequences.
Journal ArticleDOI
Crystal structure of a CAP-DNA complex: the DNA is bent by 90 degrees.
TL;DR: The 3 angstrom resolution crystal structure of the Escherichia coli catabolite gene activator protein (CAP) complexed with a 30-base pair DNA sequence shows that the DNA is bent by 90 degrees.
References
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Journal ArticleDOI
Genetic regulation: the Lac control region.
TL;DR: Identification of mutant variants of the sequence combined with the in vitro biochemical studies of others has allowed us to tentatively identify the recognition site for each of these proteins, and to suggest how CAP might act at a distance to affect the interaction of RNA polymerase with the promoter.
Journal ArticleDOI
Autoregulation and function of a repressor in bacteriophage lambda.
Mark Ptashne,Keith C. Backman,MZ Humayun,Andrea Jeffrey,Russell Maurer,Barbara J. Meyer,Robert T. Sauer +6 more
Journal ArticleDOI
DNA sequence for a low-level promoter of the lac repressor gene and an ‘up’ promoter mutation
TL;DR: The promoter for a weakly expressed constitutive gene, the lactose repressor gene (lacI), has been sequenced, along with an ‘up’ promoter mutation Iq, which results in a 10-fold enhancement in I expression in Iq.
Journal ArticleDOI
Dual control for transcription of the galactose operon by cyclic AMP and its receptor protein at two interspersed promoters
TL;DR: Results demonstrate the existence of two initiation sites, S 1 and S 2, for transcription of the galactose operon of E. coli and suggest that when CRP or cAMP is absent, transcription starts from S 2 in vivo.
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