Journal ArticleDOI
Effect of modified membrane vesicles from seminal plasma on the fertilizing capacity of rabbit spermatozoa.
B.K. Davis,B.J. Hungund +1 more
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Partial extraction of cholesterol and phospholipid from membrane vesicles in rabbit seminal plasma decreased their inhibitory effect on fertilizing capacity in rabbit spermatozoa.About:
This article is published in Biochemical and Biophysical Research Communications.The article was published on 1976-04-19. It has received 33 citations till now. The article focuses on the topics: Sperm plasma membrane & Vesicle.read more
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Current status of sperm cryopreservation: why isn't it better?
TL;DR: In this article, the authors reviewed aspects of sperm cryopreservation paralleled by events of capacitation and evaluated the possible roles of sperm membrane cholesterol, reactive oxygen species, and seminal plasma as mediators of sperm function.
Journal ArticleDOI
Development changes occurring in the lipids of ram epididymal spermatozoa plasma membrane.
John E. Parks,Roy H. Hammerstedt +1 more
TL;DR: Ram spermatozoa were obtained from different regions of the epididymis and their plasma membrane was removed using a nitrogen cavitation treatment and the molar ratio of cholesterol to phospholipid increased in the plasma membrane during maturation.
Journal ArticleDOI
Studies on the mechanism of capacitation. II. Evidence for lipid transfer between plasma membrane of rat sperm and serum albumin during capacitation in vitro.
TL;DR: The results are interpreted as broadly supporting the previous proposal that lipid exchange between albumin and sperm cells is implicated in sperm capacitation in vitro and compatible with the idea that a decreased cholesterol/phospholipid ratio in the sperm plasma membrane facilitates this transformation.
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An Mg2+ and Ca2+-stimulated adenosine triphosphatase in human prostatic fluid--part II.
TL;DR: Das ATPase-Enzymsystem wurde charakterisiert; die scheinbare Michaelis-Konstante sowie Vmax der Mg2+-abhangigen ATPase ergab 7,1 × 10−4 M bzw.
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Effect of bovine oviductal estrus-associated protein on the ability of sperm to capacitate and fertilize oocytes.
TL;DR: Bovine EAP, derived from both ODF and in vitro cultures, promotes sperm fertilizing ability and is significantly greater than that for sperm incubated without EAP or with anti-EAP.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
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Phosphorus Assay in Column Chromatography
TL;DR: If the highest accuracy was not required, the following manipulations simplified and speeded multiple total phosphorus determinations on the eluates from column chromatographic separations.
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The thiobarbituric acid assay of sialic acids.
TL;DR: This chapter discusses the different aspects of thiobarbituric acid assay of sialic acid, which is suitable for measuring the release of bound sialoic acid by sialidase and hydrolysis of sIALic acid-containing material must be carried out for the measurement of total sialsic acids.