Journal ArticleDOI
Efficient DNA-Catalyzed Porphyrin Metalation for Fluorescent Ratiometric Pb2+ Detection.
TLDR
This work has established a new DNA-based reaction that can be used for Pb2+ detection, and it also provides a highly efficient new DNAzyme for por-phyrin metalation, which might be use for signal production for other biosensors.Abstract:
Developing biosensors for Pb2+ is an important analytical topic. DNA-based Pb2+ sensors have been designed mainly based on RNA-cleaving DNAzymes and Pb2+-induced folding of G-quadruplex (G4) DNA. Porphyrin metalation is a key reaction in biology and catalysis. Many enzyme mimics have been developed to catalyze this reaction, and some metalation DNAzymes were reported with a G4 structure. Inspired by the excellent G4 binding properties of certain divalent metal ions, we herein screened a few metals and G-rich DNA sequences. The metalation activity of a DNA named T30695 (sequence: (G3T)4) was significantly accelerated by Pb2+. The reaction of Cu2+ insertion into the mesoporphyrin IX had a kcat of 0.89 min-1 and a Km of 9.8 μM, representing a catalytic efficiency similar to that of human ferrochelatase. The reason for the acceleration was attributed to Pb2+ binding of the G4 DNA and the catalytic activity of the large Pb2+ ion for this reaction. A ratiometric sensor for Pb2+ was developed by inserting Zn2+ with a detection limit of 23.5 nM Pb2+. This work has established a new DNA-based reaction that can be used for Pb2+ detection, and it also provides a highly efficient new DNAzyme for porphyrin metalation, which might be used for signal production for other biosensors.read more
Citations
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Journal ArticleDOI
Biosensing with DNAzymes
TL;DR: A comprehensive review of biosensing with DNAzymes, providing an overview of different sensing applications while highlighting major progress and seminal contributions to the field of portable biosensor devices and point-of-care diagnostics is provided in this paper.
Journal ArticleDOI
Catalytic Nucleic Acids: Biochemistry, Chemical Biology, Biosensors, and Nanotechnology
Lingzi Ma,Juewen Liu +1 more
TL;DR: A historic view is taken to summarize a few main interdisciplinary areas of research on nucleic acid enzymes that may have broader impacts on the search for new DNAzymes and ribozymes.
Journal ArticleDOI
Nucleic Acids Analysis
Yongxi Zhao,Xiaolei Zuo,Qian Li,Feng Chen,Yan-Ru Chen,Jinqi Deng,Da Han,Changlong Hao,Fujian Huang,Yanyi Huang,Guoliang Ke,Hua Kuang,Fan Li,Jiang Li,Min Li,Na Li,Zhenyu Lin,Dingbin Liu,Juewen Liu,Libing Liu,Xiaoguo Liu,Chun-Hua Lu,Fang Luo,Xiuhai Mao,Jiashu Sun,Bo Tang,Fei Wang,Jianbin Wang,Lihua Wang,Shu Wang,Lingling Wu,Zai-Sheng Wu,Fan Xia,Chuanlai Xu,Yang Yang,Bi-Feng Yuan,Quan Yuan,Chao Zhang,Zhi Zhu,Chaoyong Yang,Chaoyong Yang,Xiao-Bing Zhang,Huanghao Yang,Weihong Tan,Weihong Tan,Chunhai Fan +45 more
TL;DR: The representative strategies for the development of new nucleic acids analysis in this field are summarized, and key advantages and possible limitations are discussed.
Journal ArticleDOI
G-quadruplex DNA for construction of biosensors
TL;DR: G-quadruplex (G4) DNA has attracted growing interest since it can be used for both target recognition and signal transduction as discussed by the authors, and it has been widely used for its stable chemical properties, cost-effectiveness, and easy modification.
Journal ArticleDOI
Preparation of intelligent DNA hydrogel and its applications in biosensing
TL;DR: The preparation methods of different types of Intelligent DNA hydrogel, the applications and prospect of intelligent DNA Hydrogel in the field of biosensing are reviewed.
References
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Small molecule-based ratiometric fluorescence probes for cations, anions, and biomolecules
TL;DR: An overview of the design principles underlying small fluorescent probes that have been applied to the ratiometric detection of various analytes, including cations, anions, and biomolecules in solution and in biological samples are provided.
Journal ArticleDOI
Programmable materials and the nature of the DNA bond
TL;DR: The two main approaches to creating stiff bonds, based on DNA-based materials synthesis, are reviewed, offering perhaps the most versatile way of organizing optically active materials into architectures that exhibit unusual and deliberately tailorable plasmonic and photonic properties.