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Enzyme catalysis in organic synthesis : a comprehensive handbook
Karlheinz Drauz,Herbert Waldmann +1 more
TLDR
A tabular survey of commercially available enzymes can be found in this paper, with a focus on enzymes that are used in organic synthesis, such as: production and isolation of enzymes, immobilization of enzymes reaction techniques, use of growing or resting cells, applications of enqumes in organically synthesized organic synthesis hydrolysis and formation of C-O bonds, C-N bonds, P-O bond formation, reduction reactions oxidation reactions isomerizations introduction and removal of protecting groups extremophiles catalytic antibodies enqumatic analysis and biosensors prtoein engineeringAbstract:
Production and isolation of enzymes immobilization of enzymes reaction techniques use of growing or resting cells applications of enqumes in organic synthesis hydrolysis and formation of C-O bonds, C-N bonds, P-O bonds formation of C-C bonds reduction reactions oxidation reactions isomerizations introduction and removal of protecting groups extremophiles catalytic antibodies enqumatic analysis and biosensors prtoein engineering tabular survey of commercially available enzymes.read more
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Macroporous poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate) resins-Versatile immobilization supports for biocatalysts
TL;DR: In this paper, the influence of the resin properties on the loading of Candida antarctica lipase B (Cal-B) during immobilization and on the hydrolytic (hydrolysis of para-nitrophenyl acetate) and synthetic (ring-opening polymerization of e-caprolactone) activity of the immobilized CalB were studied.
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Preparative Biotransformations: Oxidation of Alcohols
TL;DR: In this paper, a review of the preparation methods for the oxidation of primary and secondary alcohols can be found, highlighting the advantages and the limitations of classical and innovative bioprocesses, as well as suggestions for combining recent improvements of the techniques used in biocatalysis (high throughput screening, (meta)genomics, protein engineering, metabolic engineering, and bio-process engineering) with green and efficient synthetic approaches.
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Many pathways in laboratory evolution can lead to improved enzymes: how to escape from local minima.
TL;DR: All 24 pathways were found to provide improved mutants with notably enhanced stereoselectivity, and when a library failed to contain any hits, non‐improved or even inferior mutants were used as templates in the continuation of the evolutionary pathway, thereby escaping from the local minimum.
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Shedding light on the efficacy of laboratory evolution based on iterative saturation mutagenesis
TL;DR: In this paper, an iterative saturation mutagenesis (ISM) approach is proposed to enhance the enantioselectivity and/or the thermostability of enzymes, using reduced amino acid alphabets as defined by appropriate codon degeneracies supplements.
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Directed evolution of enantioselective hybrid catalysts: a novel concept in asymmetric catalysis
Manfred T. Reetz,Martin Rentzsch,Andreas Pletsch,Matthias Maywald,Peter Maiwald,Jérôme J.-P. Peyralans,Andrea Maichele,Yu Fu,Ning Jiao,Frank Hollmann,Régis Mondière,Andreas Taglieber +11 more
TL;DR: The concept of directed evolution of enantioselective hybrid catalysts was proposed in 2001/2002 and implemented experimentally for the first time in a proof-of-concept study in 2006 as discussed by the authors.