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Enzyme catalysis in organic synthesis : a comprehensive handbook
Karlheinz Drauz,Herbert Waldmann +1 more
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TLDR
A tabular survey of commercially available enzymes can be found in this paper, with a focus on enzymes that are used in organic synthesis, such as: production and isolation of enzymes, immobilization of enzymes reaction techniques, use of growing or resting cells, applications of enqumes in organically synthesized organic synthesis hydrolysis and formation of C-O bonds, C-N bonds, P-O bond formation, reduction reactions oxidation reactions isomerizations introduction and removal of protecting groups extremophiles catalytic antibodies enqumatic analysis and biosensors prtoein engineeringAbstract:
Production and isolation of enzymes immobilization of enzymes reaction techniques use of growing or resting cells applications of enqumes in organic synthesis hydrolysis and formation of C-O bonds, C-N bonds, P-O bonds formation of C-C bonds reduction reactions oxidation reactions isomerizations introduction and removal of protecting groups extremophiles catalytic antibodies enqumatic analysis and biosensors prtoein engineering tabular survey of commercially available enzymes.read more
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Journal ArticleDOI
Lipase catalysed Michael addition of secondary amines to acrylonitrile
TL;DR: A new enzymatic process is described that widens the applicability of these biocatalysts in organic synthesis by catalyse Michael-type addition of secondary amines to acrylonitrile.
Journal ArticleDOI
New methods for the high-throughput screening of enantioselective catalysts and biocatalysts
Journal ArticleDOI
Artificial metalloenzymes based on biotin-avidin technology for the enantioselective reduction of ketones by transfer hydrogenation
TL;DR: A combined chemo-genetic procedure allows us to optimize the activity and selectivity of these hybrid catalysts: up to 94% enantiomeric excess for the reduction of p-methylacetophenone.
Patent
Synthetic ligation reassembly in directed evolution
TL;DR: In this paper, a process of preparing nucleic acid and poly nucleotide libraries by cloning non-randomly generated nucleotide fragments having ligatable ends into an expression vector was proposed.
Journal ArticleDOI
Directed evolution and the creation of enantioselective biocatalysts.
TL;DR: This work discusses recent examples of creating enantioselective biocatalysts by directed evolution and identifies improved enzyme variants identified, preferably after being secreted into culture medium, by screening or selection for the desired property.