Open AccessJournal Article
Establishment and characterization of a human prostatic carcinoma cell line (PC-3).
TLDR
The establishment, characterization, and tumorigenicity of a new epithelial cell line (PC-3) from a human prostatic adenocarcinoma metastatic to bone is reported, which should be useful in investigating the biochemical changes in advanced prostatic cancer cells and in assessing their response to chemotherapeutic agents.Abstract:
The establishment, characterization, and tumorigenicity of a new epithelial cell line (PC-3) from a human prostatic adenocarcinoma metastatic to bone is reported The cultured cells show anchorage-independent growth in both monolayers and in soft agar suspension and produce subcutaneous tumors in nude mice Culture of the transplanted tumor yielded a human cell line with characteristics identical to those used initially to produce the tumor PC-3 has a greatly reduced dependence upon serum for growth when compared to normal prostatic epithelial cells and does not respond to androgens, glucocorticoids, or epidermal or fibroblast gowth factors Karyotypic analysis by quinacrine banding revealed the cells to be completely aneuploid with a modal chromosome number in the hypotriploid range At least 10 distinctive marker chromosomes were identified The overall karyotype as well as the marker chromosomes are distinct from those of the HeLa cell Electron microscopic studies revealed many features common to neoplastic cells of epithelial origin including numerous microvilli, junctional complexes, abnormal nuclei and nucleoli, abnormal mitochondria, annulate lamellae, and lipoidal bodies Overall, the functional and morphologic characteristics of PC-3 are those of a poorly-differentiated adenocarcinoma These cells should be useful in investigating the biochemical changes in advanced prostatic cancer cells and in assessing their response to chemotherapeutic agentsread more
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Disparities in prostate cancer incidence and mortality rates: Solvable or not?
Simone Badal,William Aiken,Belinda F. Morrison,Henkel Valentine,Sophia Bryan,Andrew Gachii,Camille Ragin +6 more
TL;DR: Different contributing factors to the current disparities in PCa incidence and mortality rates are explored with emphasis on the incongruence in how research has been conducted in understanding the disease towards developing therapies.
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Biosynthesis of the carbohydrate antigenic determinants, Globo H, blood group H, and Lewis b: a role for prostate cancer cell α1,2-L-fucosyltransferase
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Androgen-Regulated and Highly Tumorigenic Human Prostate Cancer Cell Line Established from a Transplantable Primary CWR22 Tumor
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TL;DR: This androgen-regulated and tumorigenic human prostate cancer cell line provides a valuable tool for studies on androgen regulation of prostate cancer cells and on the molecular mechanisms taking place in growth promotion of prostatecancer when androgens are withdrawn from the growth environment.
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NES1/KLK10 gene represses proliferation, enhances apoptosis and down-regulates glucose metabolism of PC3 prostate cancer cells.
Jiajia Hu,Hu Lei,Xiaochun Fei,Sheng Liang,Hanzhang Xu,Dongjun Qin,Yue Wang,Ying-Li Wu,Li Biao +8 more
TL;DR: It is demonstrated that KLK10 may function as a tumour suppressor by repressing proliferation, enhancing apoptosis and decreasing glucose metabolism in PC3 cells.
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cAMP induced modifications of HOX D gene expression in prostate cells allow the identification of a chromosomal area involved in vivo with neuroendocrine differentiation of human advanced prostate cancers.
Monica Cantile,Annamaria Kisslinger,Luca Cindolo,Giulia Schiavo,Vincenzo D'Antò,Renato Franco,Vincenzo Altieri,Antonio Gallo,A. Villacci,Donatella Tramontano,Clemente Cillo +10 more
TL;DR: The in vivo expression of Neuro D1 in human advanced prostate cancers correlate with the state of tumor differentiation as measured by Gleason score, suggesting that the chromosomal area 2q 31‐33 might be involved in the epithelial‐ND characteristic of humanAdvanced prostate cancers.