Open AccessJournal Article
Establishment and characterization of a human prostatic carcinoma cell line (PC-3).
TLDR
The establishment, characterization, and tumorigenicity of a new epithelial cell line (PC-3) from a human prostatic adenocarcinoma metastatic to bone is reported, which should be useful in investigating the biochemical changes in advanced prostatic cancer cells and in assessing their response to chemotherapeutic agents.Abstract:
The establishment, characterization, and tumorigenicity of a new epithelial cell line (PC-3) from a human prostatic adenocarcinoma metastatic to bone is reported The cultured cells show anchorage-independent growth in both monolayers and in soft agar suspension and produce subcutaneous tumors in nude mice Culture of the transplanted tumor yielded a human cell line with characteristics identical to those used initially to produce the tumor PC-3 has a greatly reduced dependence upon serum for growth when compared to normal prostatic epithelial cells and does not respond to androgens, glucocorticoids, or epidermal or fibroblast gowth factors Karyotypic analysis by quinacrine banding revealed the cells to be completely aneuploid with a modal chromosome number in the hypotriploid range At least 10 distinctive marker chromosomes were identified The overall karyotype as well as the marker chromosomes are distinct from those of the HeLa cell Electron microscopic studies revealed many features common to neoplastic cells of epithelial origin including numerous microvilli, junctional complexes, abnormal nuclei and nucleoli, abnormal mitochondria, annulate lamellae, and lipoidal bodies Overall, the functional and morphologic characteristics of PC-3 are those of a poorly-differentiated adenocarcinoma These cells should be useful in investigating the biochemical changes in advanced prostatic cancer cells and in assessing their response to chemotherapeutic agentsread more
Citations
More filters
Journal ArticleDOI
Androgens down-regulate the expression of the human homologue of paternally expressed gene-3 in the prostatic adenocarcinoma cell line LNCaP.
TL;DR: Both androgens and loss of differentiation may affect the expression of Peg3, a mediator of the effects of TNF, and further experiments will be required to explore whether these changes affect the responsiveness of prostate tumor cells to TNF.
Journal ArticleDOI
Human Tumor Cell Proliferation Evaluated Using Manganese-Enhanced MRI
TL;DR: These data clearly demonstrate the tumor-cell dependent nature of the relationship between proliferation and calcium influx, and underscore the usefulness of MEMRI as a non-invasive method for investigating this link.
Journal ArticleDOI
Identifying density-dependent interactions in collective cell behaviour.
TL;DR: The results provide strong evidence to suggest that motility is density-dependent in scratch assays and show that standard modelling approaches cannot simultaneously describe data initiated across a range of initial densities, the opposite of the assumptions in standard continuum models.
Journal ArticleDOI
Analytical validation of a real-time reverse transcription-polymerase chain reaction quantitation of different transcripts of the Wilms' tumor suppressor gene (WT1).
Catherine I. Dumur,Chavaboon Dechsukhum,David S. Wilkinson,Carleton T. Garrett,Joy L. Ware,Andrea Ferreira-Gonzalez +5 more
TL;DR: Analytical validation of a real-time RT-PCR assay for the absolute quantitation of two different WT1 transcripts with specific primers and probes that ensure specificity for each WT1 variant is reported.
Journal ArticleDOI
Changes in mitochondrial function induced by dequalinium precede oxidative stress and apoptosis in the human prostate-cancer cell line PC-3
K. Makowska,María Cristina Estañ,Irene Ganan-Gomez,María del Carmen Boyano-Adánez,Ana I. García-Pérez,Pilar Sancho +5 more
TL;DR: DQA was shown to cause moderate to strong cell death in a time and concentration dependent manner, causing a most advantageous effect at a concentration of 10 μM applied for a long 48 h time period, which might be a consequence of the kinetics of intracellular DQA accumulation in mitochondria, but also of the mechanisms of D QA-induced cell death.