Estimating Nuclear DNA Content in Peach and Related Diploid Species Using Laser Flow Cytometry and DNA Hybridization
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A relatively small and stable nuclear genome typifies the Prunus species investigated, consistent with their low, basic chromosome number (x = 8).Citations
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AC/GT and AG/CT microsatellite repeats in peach [Prunus persica (L) Batsch]: isolation, characterisation and cross-species amplification in Prunus.
TL;DR: Because of their appreciable polymorphism and wide cross-species transportability, most of these new markers can be integrated into the linkage maps which are currently being constructed in peach, as well as in other stone fruit crops, such as almond, apricot, cherry and plum.
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Two new nuclear isolation buffers for plant DNA flow cytometry: a test with 37 species.
TL;DR: WPB is a reliable buffer which is also suitable for the analysis of problematic tissues/species, although GPB failed with some plant species, it provided high-quality DNA histograms in species from which nuclear suspensions are easy to prepare.
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Characterization of microsatellite markers in peach (Prunus persica (L.) Batsch)
Bryon Sosinski,M. Gannavarapu,L. D. Hager,L. E. Beck,Graham J.W. King,C. D. Ryder,S. Rajapakse,Wm. Vance Baird,R E Ballard,Albert G. Abbott +9 more
TL;DR: Estimates of microsatellite frequencies were determined, and indicate that CT repeats occur every 100 kb, CA repeats every 420 kb, and AGG repeats every 700 kb in the peach genome.
TECHNICAL ARTICLE Two New Nuclear Isolation Buffers for Plant DNA Flow Cytometry: A Test with 37 Species
TL;DR: In this paper, the performance of general purpose buffer (GPB) and woody plant buffer (WPB) for plant DNA histogram analysis was evaluated in a set of 37 plant species.
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Multiple Models for Rosaceae Genomics
Vladimir Shulaev,Schuyler S. Korban,Bryon Sosinski,Albert G. Abbott,Herb S. Aldwinckle,Kevin M. Folta,Amy Iezzoni,Dorrie Main,Pere Arús,Abhaya M. Dandekar,Kim S. Lewers,Susan K. Brown,Thomas M. Davis,Susan E. Gardiner,Daniel Potter,Richard E. Veilleux +15 more
TL;DR: This report serves as a synopsis of the resources and initiatives of the Rosaceae community, recent developments in Rosaceae genomics, and plans to apply newly accumulated knowledge and resources toward breeding and crop improvement.
References
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A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
TL;DR: A technique for conveniently radiolabeling DNA restriction endonuclease fragments to high specific activity is described, and these "oligolabeled" DNA fragments serve as efficient probes in filter hybridization experiments.
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
TL;DR: In this article, a technique for conveniently radiolabeling DNA restriction endonuclease fragments to high specific activity is described, where DNA fragments are purified from agarose gels directly by ethanol precipitation and are then denatured and labeled with the large fragment of DNA polymerase I, using random oligonucleotides as primers.
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Nuclear DNA content of some important plant species
K. Arumuganathan,E. D. Earle +1 more
TL;DR: This survey identified several horticultural crops in a variety of families with genomes only two or three times as large asArabidopsis and several fruit trees (a pricot, cherry, mango, orange, papaya, and peach) that should facilitate molecular studies of these crops.
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Rapid flow cytometric analysis of the cell cycle in intact plant tissues.
David W. Galbraith,Kristi R. Harkins,Joyce M. Maddox,Nicola M. Ayres,Dharam P. Sharma,Ebrahim Firoozabady +5 more
TL;DR: The amount of nuclear DNA in the homogenates of monocotyledonous and dicotylingonous plants was accurately and rapidly determined by flow microfluorometry, and the distribution of nuclei involved in the cell cycle was charted for tissues selected from different physical locations or developmental stages.
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Nuclear DNA Amounts in Angiosperms
Michael D. Bennett,J. B. Smith +1 more
TL;DR: This paper lists absolute nuclear DNA amounts for 753 angiosperm species, primarily for reference purposes, and so the species are listed in alphabetical order, as this was felt to be more helpful to cyto- and biochemists whom, it is anticipated, will be among its major users.