Extended focused image in microscopy by digital Holography.
Pietro Ferraro,Simonetta Grilli,Domenico Alfieri,Sergio De Nicola,Andrea Finizio,Giovanni Pierattini,Bahram Javidi,Giuseppe Coppola,V. Striano +8 more
TLDR
It is demonstrated that an extended focused image of an object can be obtained through digital holography without any mechanical scanning or special optical components.Abstract:
In microscopy, high magnifications are achievable for investigating micro-objects but the paradigm is that higher is the required magnification, lower is the depth of focus. For an object having a three-dimensional (3D) complex shape only a portion of it appears in good focus to the observer who is essentially looking at a single image plane. Actually, two approaches exist to obtain an extended focused image, both having severe limitations since the first requires mechanical scanning while the other one requires specially designed optics. We demonstrate that an extended focused image of an object can be obtained through digital holography without any mechanical scanning or special optical components. The conceptual novelty of the proposed approach lies in the fact that it is possible to completely exploit the unique feature of DH in extracting all the information content stored in hologram, amplitude and phase, to extend the depth of focus.read more
Citations
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Journal ArticleDOI
Principles and techniques of digital holographic microscopy
TL;DR: Digital holography is an emerging field of new paradigm in general imaging applications as discussed by the authors, and a review of a subset of the research and development activities in digital holographic microscopy techniques and applications is presented.
Journal ArticleDOI
Optical Techniques for Information Security
TL;DR: An overview of the potential of free space optical technology in information security, encryption, and authentication is presented and optical authentication techniques applied to ID tags with visible and near infrared imaging are reviewed.
Book ChapterDOI
Quantitative Phase Imaging
PatentDOI
Extended depth of field imaging for high speed object analysis
William E. Ortyn,David Basiji,Keith Frost,Luchuan Liang,Richard Bauer,Brian E. Hall,David J. Perry +6 more
TL;DR: In this article, an optical distortion element is introduced into the flow imaging system, such that a point spread function (PSF) of the imaging system is invariant across an extended depth of field.
Patent
Blood and cell analysis using an imaging flow cytometer
William E. Ortyn,David Basiji,Philip J. Morrissey,Thaddeus C. George,Brian E. Hall,Cathleen A. Zimmerman,David J. Perry +6 more
TL;DR: In this article, multimodal/multispectral images of a population of cells are simultaneously collected and morphometric and/or morphometric features identifiable in the images are used to separate the population into a plurality of subpopulations.
References
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Extended depth of field through wave-front coding
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Ulf Schnars,Werner Jüptner +1 more
TL;DR: The principles and major applications of digital recording and numerical reconstruction of holograms (digital holography) are described, which are applied to measure shape and surface deformation of opaque bodies and refractive index fields within transparent media.
Journal ArticleDOI
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TL;DR: In this paper, it is shown that a coherent monochromatic wave can be used to reconstruct the original image of an object from its mirror image with respect to the coherent background.
Journal ArticleDOI
Digital Image Formation From Electronically Detected Holograms
Joseph W. Goodman,R. W. Lawrence +1 more
TL;DR: In high precision holographic imagery of weak objects of small angular subtense, electronic detection and digital image formation have distinct advantages as mentioned in this paper, and experiments with a vidicon detector and a PDP-6 computer have yielded reconstructed images of good quality with computation times of five minutes.