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Extracellular Biosynthesis of Gold Nanoparticles using Aspergillus niger – its Characterization and Stability

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TLDR
Different characterization studies showed that the extracellular enzyme secreted by Aspergillus niger NCIM 616 might be responsible for both formation and capping of the metal nanoparticles.
Abstract
The development of an eco-friendly and reliable process for the synthesis of gold nanomaterials using microorganisms is gaining importance in the field of nanotechnology. In the present study, gold nanoparticles have been synthesized by reduction of aqueous gold ions using the culture supernatant of Aspergillus niger NCIM 616. The synthesis of the gold nanoparticles was monitored by UV-visible spectroscopy. The particles thereby obtained were characterized by UV, Fourier transform infrared (FTIR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-ray diffraction (XRD). The stability of the synthesized gold nanoparticles was analyzed by zeta potential measurement. Treatment of the fungal supernatant with aqueous Au+ ions produced nanoparticles with an average particle size of 12.79 ± 5.61 nm. Different characterization studies showed that the extracellular enzyme secreted by Aspergillus niger NCIM 616 might be responsible for both formation and capping of the metal nanoparticles.

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Biosynthesis of nanoparticles using microbes- a review.

TL;DR: The FTIR study reveals that diverse biomolecules viz. carboxyl group, primary and secondary amines, amide I, II, and III bands etc serve as a tool for bioreduction and capping agents there by offering stability to particles by preventing agglomeration and growth.
Journal ArticleDOI

Nanoparticles Biosynthesized by Fungi and Yeast: A Review of Their Preparation, Properties, and Medical Applications.

TL;DR: A short summary of the present study universally on the utilization of eukaryotes like yeast and fungi in the biosynthesis of nanoparticles (NPs) and their uses is provided.
Journal ArticleDOI

Fungal biosynthesis of gold nanoparticles: mechanism and scale up

TL;DR: The known mechanisms for Au NP biosynthesis in viable fungi and fungal protein extracts are described and the most suitable bioreactors for industrial AuNP biosynthesis are discussed.
Journal ArticleDOI

Fabrication of Metal Nanoparticles from Fungi and Metal Salts: Scope and Application

TL;DR: This review focuses on the biogenic synthesis of metal nanoparticles by fungi to explore the chemistry of their formation extracellularly and intracellularly.
Journal ArticleDOI

Combined efficacy of biologically synthesized silver nanoparticles and different antibiotics against multidrug-resistant bacteria.

TL;DR: Overall, the synergistic effect of antibiotics and nanoparticles resulted in a 0.2–7.0 fold-area increase in antibacterial activity, which clearly revealed that nanoparticles can be effectively used in combination with antibiotics in order to improve their efficacy against various pathogenic microbes.
References
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Journal ArticleDOI

Shape-Controlled Synthesis of Gold and Silver Nanoparticles

TL;DR: Monodisperse samples of silver nanocubes were synthesized in large quantities by reducing silver nitrate with ethylene glycol in the presence of poly(vinyl pyrrolidone) (PVP), characterized by a slightly truncated shape bounded by {100, {110}, and {111} facets.
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Surface Plasmon Spectroscopy of Nanosized Metal Particles

TL;DR: In this paper, the use of optical measurements to monitor electrochemical changes on the surface of nanosized metal particles is discussed within the Drude model, and the absorption spectrum of a metal sol in water is shown to be strongly affected by cathodic or anodic polarization, chemisorption, metal adatom deposition, and alloying.
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Rapid synthesis of Au, Ag, and bimetallic Au core-Ag shell nanoparticles using Neem (Azadirachta indica) leaf broth.

TL;DR: The rates of reduction of the metal ions by Neem leaf extract are much faster than those observed by us in earlier studies using microorganisms such as fungi, highlighting the possibility that nanoparticle biological synthesis methodologies will achieve rates of synthesis comparable to those of chemical methods.
Journal ArticleDOI

Extracellular biosynthesis of silver nanoparticles using the fungus Fusarium oxysporum

TL;DR: It is observed that aqueous silver ions when exposed to the fungus Fusarium oxysporum are reduced in solution, thereby leading to the formation of an extremely stable silver hydrosol, creating the possibility of developing a rational, fungal-based method for the synthesis of nanomaterials over a range of chemical compositions, which is currently not possible by other microbe-based methods.
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Toxicity of Gold Nanoparticles Functionalized with Cationic and Anionic Side Chains

TL;DR: Concentration-dependent lysis mediated by initial electrostatic binding was observed in dye release studies using lipid vesicles, providing the probable mechanism for observed toxicity with the cationic MMPCs.
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