First time β-farnesene production by the versatile bacterium Cupriavidus necator.
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In this paper, the authors describe β-farnesene production by the versatile bacterial host Cupriavidus necator on fructose, which is known to grow hetero-and autotrophically and even in bioelectrochemical systems.Abstract:
Terpenes are remarkably diverse natural structures, which can be formed via two different pathways leading to two common intermediates. Among those, sesquiterpenes represent a variety of industrially relevant products. One important industrially produced product is β-farnesene as a precursor for a jet fuel additive. So far, microbial terpene production has been mostly limited to known production hosts, which are only able to grow on heterotrophic substrates. In this paper, we for the first time describe β-farnesene production by the versatile bacterial host Cupriavidus necator on fructose, which is known to grow hetero- and autotrophically and even in bioelectrochemical systems. We were able to show a growth-dependent production of β-farnesene by expressing the β-farnesene synthase from Artemisia annua in C. necator H16 PHB-4. Additionally, we performed a scale-up in a parallel reactor system with production titers of 26.3 ± 1.3 µM β-farnesene with a fed-batch process. The β-farnesene production titers reported in this paper are not in the same range as titers published with known heterotrophic producers E. coli or S. cerevisiae. However, this proof-of-principle study with C. necator as production host opens new synthesis routes toward a sustainable economy and leaves room for further optimizations, which have been already performed with the known production strains.read more
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Progress and perspectives for microbial production of farnesene
TL;DR: In this article , a review summarized these strategies, including regulation of farnesene synthesis pathways, and proposed some emerging tools and methods in stain engineering, and then future farnesenesene biosynthesis challenges were discussed.
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Upgrading Kolbe Electrolysis—Highly Efficient Production of Green Fuels and Solvents by Coupling Biosynthesis and Electrosynthesis
TL;DR: In this paper , the coupling of the Kolbe electrolysis at the anode with a subsequent microbial conversion of the cathodically produced co-product hydrogen was shown for the first time, and the implementation of a paired electrolyser resulted in superior process performances with overall efficiencies of up to 64.4
Journal ArticleDOI
Synthetic biology toolkit for engineering Cupriviadus necator H16 as a platform for CO2 valorization.
TL;DR: In this paper, a review of C. necator H16, including genetic engineering vectors, heterologous gene expression elements, platform strain and genome engineering, and transformation strategies, is presented.
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Nitrogen‐containing Polymers Derived from Terpenes: Possibilities and Limitations
Journal ArticleDOI
Upgrading Kolbe Electrolysis—Highly Efficient Production of Green Fuels and Solvents by Coupling Biosynthesis and Electrosynthesis
TL;DR: In this article , the coupling of the Kolbe electrolysis at the anode with a subsequent microbial conversion of the cathodically produced co-product hydrogen was shown for the first time, which resulted in superior process performances with overall efficiencies of up to 64.4 %.
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