Proceedings ArticleDOI
Fluorescent molecular probes based on excited state prototropism in lipid bilayer membrane
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TLDR
The recent work using 1-naphthol as an ESPT fluorescent molecular probe has shown that the incorporation of monomeric bile salt molecules into lipid bilayer membranes composed from dipalmitoylphosphatidylcholine and dimyristoy lphosph atidyl choline induce appreciable wetting of the bilayer up to the hydrocarbon core region, even at very low concentrations of the bile salts.Abstract:
Excited state prototropism (ESPT) is observed in molecules having one or more ionizable protons, whose proton
transfer efficiency is different in ground and excited states. The interaction of various ESPT molecules like naphthols
and intramolecular ESPT (ESIPT) molecules like hydroxyflavones etc. with different microheterogeneous media have
been studied in detail and excited state prototropism as a probe concept has been gaining ground. The fluorescence of
different prototropic forms of such molecules, on partitioning to an organized medium like lipid bilayer membrane,
often show sensitive response to the local environment with respect to the local structure, physical properties and
dynamics. Our recent work using 1-naphthol as an ESPT fluorescent molecular probe has shown that the incorporation
of monomeric bile salt molecules into lipid bilayer membranes composed from dipalmitoylphosphatidylcholine (DPPC,
a lung surfactant) and dimyristoylphosphatidylcholine (DMPC), in solid gel and liquid crystalline phases, induce
appreciable wetting of the bilayer up to the hydrocarbon core region, even at very low (≤ 1 mM) concentrations of the
bile salts. The incorporation and location of fisetin, an ESIPT molecule having antioxidant properties, in lipid bilayer
membrane has been sensitively monitored from its intrinsic fluorescence behaviour.read more
Citations
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Journal ArticleDOI
Photophysical behavior of 8-anilino-1-naphthalenesulfonate in vesicles of pulmonary surfactant dipalmitoylphosphatidylcholine (DPPC) and its sensitivity toward the bile salt-vesicle interaction.
TL;DR: Lower-temperature shift in the phase transition of DPPC bilayer indicates that fluorescence anisotropy of ANS is sensitive enough to the bile salt induced perturbation in the packed acyl chains of DP PC bilayer and modification in the membrane fluidity.
References
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Journal ArticleDOI
Hydration dynamics of protons from photon initiated acids
TL;DR: In this paper, a Markov random walk theory was used to identify a water cluster as the effective acceptor for both naphthol and 2-naphthols, indicating that the structure H/sub 9/O/sub 4//sup +/ plays a direct role in proton hydration dynamics.
Journal ArticleDOI
Investigations on the binding and antioxidant properties of the plant flavonoid fisetin in model biomembranes.
TL;DR: The novel use of the intrinsic fluorescence characteristics of the plant flavonoid fisetin (3,3′,4′,7‐OH flavone) to explore its binding and site(s) of solubilisation in egg lecithin liposomal membranes is demonstrated.
Journal ArticleDOI
Bile salt induced solubilization of synthetic phosphatidylcholine vesicles studied by isothermal titration calorimetry
TL;DR: Isothermal titration calorimetry (ITC) was used to investigate the interactions of bile salts with phosphatidylcholine vesicles and to study the influence of the alkyl chain length of the phospholipids on the phase behavior.
Journal ArticleDOI
Phase Transitions in Phospholipid Vesicles: Excited State Prototropism of 1-Naphthol as a Novel Probe Concept
J. Sujatha,Ashok Kumar Mishra +1 more
TL;DR: In this paper, 1-Naphthol (1-ROH) undergoes partial dissociation in the excited state in liposome membrane, giving two fluorescence peaks as against a single peak in water.
Journal ArticleDOI
Photophysical Behavior of Fisetin in Dimyristoylphosphatidylcholine Liposome Membrane
TL;DR: A detailed photophysical study of the plant flavonoid fisetin in a dimyristoylphosphatidylcholine (DMPC) bilayer membrane has been carried out as mentioned in this paper.
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Bijan Kumar Paul,Nikhil Guchhait +1 more