Journal ArticleDOI
Functional significance of responsiveness to capacitating conditions in boar spermatozoa.
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TLDR
The combination of sperm responsiveness parameters applied here is a suitable tool for the evaluation of sperm function and improved the prediction level of multiple regression models for farrowing rate and litter size.About:
This article is published in Theriogenology.The article was published on 2005-11-01. It has received 45 citations till now. The article focuses on the topics: Sperm & Capacitation.read more
Citations
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Influence of ejaculation time on sperm quality parameters in high performance boars
Guilherme Oberlender,Luis David Solis Murgas,Márcio Gilberto Zangeronimo,Adriana Cristina da Silva,Luciano José Pereira +4 more
TL;DR: Animals with higher ejaculation times were found to have a higher semen volume and the correlation between these variables and the ejaculation time was found to be positive.
Journal ArticleDOI
Determination of a cooling-rate frame for antibiotic-free preservation of boar semen at 5°C.
Aline Fernanda Lopes Paschoal,Aline Fernanda Lopes Paschoal,Anne-Marie Luther,Helen Jäkel,Kathi Scheinpflug,Kristin Mühldorfer,Fernando Pandolfo Bortolozzo,Dagmar Waberski +7 more
TL;DR: In this paper, an optimal cooling-rate frame for boar semen preserved at 5°C in an antibiotic-free extender was determined, and the sperm motility, membrane integrity, membrane fluidity, mitochondrial membrane potential and the response to the capacitation stimulus bicarbonate remained at a high level for 144 h at 5 °C when the semen was initially cooled in a cooling rate frame ranging from 0.01 to 0.09°C min-1 in the temperature zone from 30 to 25°C.
Journal ArticleDOI
Dynamics of the induced acrosome reaction in boar sperm evaluated by flow cytometry.
TL;DR: Both sperm viability and the induced AR were significantly affected by sperm capacitation, incubation time and ionophore concentration, and suggestions for an optimized AR induction protocol are led to.
Optimal characteristics of spermatozoa for semen technologies in pigs.
I. Parrilla,Juan M. Vazquez,I. Caballero,Maria A. Gil,M. Hernandez,J Roca,Xiomara Lucas,Emilio A. Martinez +7 more
TL;DR: In this article, the authors summarized the value of the current available methods for their functional assessment as well as the effects of these technologies on boar sperm functionality and strategies developed to reduce sperm damage and improve the yields of both sperm technologies in swine production with particular attention to the contributions of the authors.
Journal ArticleDOI
Assessment of chilling injury in hypothermic stored boar spermatozoa by multicolor flow cytometry.
TL;DR: In this paper, the authors evaluated multiparameter flow cytometry panels to ascertain whether they are useful tools for identifying sub-lethal damage of sperm function at a single cell level, thus considering the high intrinsic sperm heterogeneity in a sample.
References
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Book
The Physiology of Reproduction
Ernst Knobil,J. D. Neill +1 more
TL;DR: The gametes, fertilization and early embryogenesis the reproductive systems - the female, the male the pituitary and the hypothalmus, and the reproductive processes and their control.
Journal ArticleDOI
Use of fluorescent probes to assess membrane integrity in mammalian spermatozoa
TL;DR: The technique appeared to provide more reliable estimations of the percentage of functional cells than did motility estimations or assessments of acrosomal integrity, and demonstrated the sensitivity of the sperm plasma membrane to cold shock: virtually all cells rapidly became permeable to the stains after such stress.
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Cholesterol efflux-mediated signal transduction in mammalian sperm: cholesterol release signals an increase in protein tyrosine phosphorylation during mouse sperm capacitation.
Pablo E. Visconti,XiaoPing Ning,Miguel W. Fornés,Juan G. Alvarez,Paula Stein,Stephanie A. Connors,Gregory S. Kopf +6 more
TL;DR: It is demonstrated that cholesterol release is associated with the activation of a transmembrane signal transduction pathway involving PK-A and protein tyrosine phosphorylation, leading to functional maturation of the sperm.
Journal ArticleDOI
Bicarbonate/CO2, an effector of capacitation, induces a rapid and reversible change in the lipid architecture of boar sperm plasma membranes
TL;DR: It is found that bicarbonate causes a rapid increase in the ability of live boar spermatozoa to bind merocyanine, apparently by perturbing enzymic control processes.
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Evaluation of in vitro capacitation of stallion spermatozoa.
TL;DR: It was found that merocyanine 540 detects capacitation-related changes much earlier than CTC does, and that flow cytometry for evaluation of capacitation and AR was a quicker and more accurate technique than fluorescence microscopy.