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Journal ArticleDOI

Galactose regulation in Saccharomyces cerevisiae: The enzymes encoded by the GAL7, 10, 1 cluster are co-ordinately controlled and separately translated

James R. Broach
- 15 Jun 1979 - 
- Vol. 131, Iss: 1, pp 41-53
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TLDR
The results suggest that the GAL 3 gene product is responsible for inducer synthesis and that the actual inducer is an intermediate in galactose metabolism.
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This article is published in Journal of Molecular Biology.The article was published on 1979-06-15. It has received 71 citations till now. The article focuses on the topics: Gene cluster & Galactokinase.

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Citations
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Journal ArticleDOI

Variety in the level of gene control in eukaryotic cells

TL;DR: Definition of eukaryotic transcription units as simple and complex affords a framework in which to discuss control at the level of RNA processing for which several examples also are known.
Journal ArticleDOI

Nutrient sensing and signaling in the yeast Saccharomyces cerevisiae

TL;DR: The yeast Saccharomyces cerevisiae has been a favorite organism for pioneering studies on nutrient-sensing and signaling mechanisms, and its discovery of nutrient transceptors (transporter receptors) as nutrient sensors has led to important new concepts and insight into nutrient-controlled cellular regulation.
Journal ArticleDOI

Use of lacZ fusions to delimit regulatory elements of the inducible divergent GAL1-GAL10 promoter in Saccharomyces cerevisiae.

TL;DR: A set of deletions that removed various portions of the GAL10 regulatory sequences from a GAL 10-CYC1-lacZ fusion was constructed in vitro and defined a relatively guanine-cytosine-rich region of 45 base pairs that contained sequences necessary for full-strength galactose induction.
Journal ArticleDOI

The organization and transcription of the galactose gene cluster of Saccharomyces

TL;DR: The transcriptional activity of a kb † region of Saccharomyces DNA centered on three galactose-inducible sequences has been examined and suggests the presence of a promoter for the transcription of the GAL1 gene, an adjacent promoter forthe transcription of GAL10 and/or both GAL8 and GAL7, and a third promoters for theGAL7 gene.
References
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Journal Article

Protein Measurement with the Folin Phenol Reagent

TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal Article

Cleavage of structural proteins during the assemble of the head of bacterio-phage T4

U. K. Laemmli
- 01 Jan 1970 - 
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
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