Journal ArticleDOI
Gene construction, expression and functional testing of an inotropic peptide from the venom of the black scorpion Hottentotta judaicus.
M. A. Tekook,Larissa Fabritz,Paulus Kirchhof,Simone König,F.U. Müller,Wilhelm Schmitz,Tzachy Tal,Eliahu Zlotkin,Uwe Kirchhefer +8 more
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TLDR
The recombinant expression of the functionally active cardiac inotropic peptide Bj-IP, a new βNaScTx from H. judaicus, is shown for promising pharmacological applications and the use of SOE-PCR may help to facilitate in future the high throughput of cloning and/or modification of scorpion toxin genes.About:
This article is published in Toxicon.The article was published on 2012-12-15. It has received 3 citations till now. The article focuses on the topics: Scorpion toxin & Recombinant DNA.read more
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Journal ArticleDOI
AdE-1, a new inotropic Na(+) channel toxin from Aiptasia diaphana, is similar to, yet distinct from, known anemone Na(+) channel toxins.
Nir Nesher,Eli Shapira,Daniel Sher,Daniel Sher,Yehu Moran,Liora Tsveyer,Ana Luiza Turchetti-Maia,Michal Horowitz,Binyamin Hochner,Eliahu Zlotkin +9 more
TL;DR: AdE-1 (ion channel modifier), a novel cardiotonic peptide from the sea anemone Aiptasia diaphana, which differs from other cnidarian toxins, and its effects on twitch relaxation velocity, action potential amplitude and on the time to peak suggest that this novel toxin affects cardiomyocyte function via a more complex mechanism.
Journal ArticleDOI
Heterologous expression and purification of neurotoxic hainantoxin-III in E. coli.
TL;DR: It was shown that the rHNTX-III was able to block currents generated by human Nav1.7 at an IC50 of 225 nM and also have high selectivity for different voltage-gated sodium channels and therefore, it has very similar activity to the natural one.
Journal ArticleDOI
First Report on an Inotropic Peptide Activating Tetrodotoxin-Sensitive, “Neuronal” Sodium Currents in the Heart
Paulus Kirchhof,Tzachy Tal,Larissa Fabritz,Jan Klimas,Nir Nesher,Jan S. Schulte,Petra Ehling,Tatayana Kanyshkova,Thomas Budde,Sigrid Nikol,Lisa Fortmueller,Birgit Stallmeyer,Frank U. Müller,Eric Schulze-Bahr,Wilhelm Schmitz,Eliahu Zlotkin,Uwe Kirchhefer +16 more
TL;DR: The pilot experiments suggest that selective activation of tetrodotoxin-sensitive neuronal sodium channels can safely increase cardiac contractility, and the peptide described here may become a lead compound for a new class of positive inotropic agents.
References
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Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
Book ChapterDOI
Use of T7 RNA polymerase to direct expression of cloned genes.
Journal ArticleDOI
Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels
TL;DR: A new modification of silver staining is presented which utilizes two chemical properties of thiosulfate: image enhancement by pretreatment of fixed gels, and formation of soluble silver complexes which prevents unspecific background staining during image development.
Journal ArticleDOI
Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension.
TL;DR: Gene splicing by overlap extension is a new approach for recombining DNA molecules at precise junctions irrespective of nucleotide sequences at the recombination site and without the use of restriction endonucleases or ligase.