Genetic relationship in the 'Bacillus cereus group' by rep-PCR fingerprinting and sequencing of a Bacillus anthracis-specific rep-PCR fragment.
Ameur Cherif,Lorenzo Brusetti,Sara Borin,Aurora Rizzi,Abdellatif Boudabous,Hala Khyami-Horani,Daniele Daffonchio +6 more
TLDR
The genetic relationship in the Bacillus cereus group is evaluated by rep‐PCR fingerprinting to evaluate the genetic relationship by checking the correspondence between the E.coli and Bacillus strains.Abstract:
Aims: To evaluate the genetic relationship in the Bacillus cereus group by rep-PCR fingerprinting.
Methods and Results: A collection of 112 strains of the six species of the B. cereus group was analysed by rep-PCR fingerprinting using the BOX-A1R primer. A relative genetic distinctness was found among the species. Cluster analysis of the rep-PCR patterns showed clusters of B. thuringiensis strains quite separate from those of B. cereus strains. The B. anthracis strains represented an independent lineage in a B. cereus cluster. The B. mycoides, B. pseudomycoides and B. weihenstephanensis strains were clustered into three groups at some distance from the other species. Comparison of sequences of AC-390, a typical B. anthracis rep-PCR fragment, from 27 strains of B. anthracis, B. cereus, B. thuringiensis and B. weihenstephanensis, representative of different clusters identified by rep-PCR fingerprinting, confirmed that B. anthracis diverges from its related species.
Conclusions: The genetic relationship deduced from the rep-PCR patterns indicates a relatively clear separation of the six species, suggesting that they can indeed be considered as separate units.
Significance and Impact of the Study: rep-PCR fingerprinting can make a contribution in the clarification of the genetic relationships between the species of the B. cereus group.read more
Citations
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From soil to gut: Bacillus cereus and its food poisoning toxins
TL;DR: The toxins associated with foodborne diseases frequently caused by B. cereus are reviewed, and recent findings regarding the associated toxins are discussed, as well as the present knowledge on virulence regulation.
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Genomics of the Bacillus cereus group of organisms
TL;DR: This group of organisms represents microbes of high economic, medical and biodefense importance and contains the highest number of closely related fully sequenced genomes, giving the unique opportunity for thorough comparative genomic analyses.
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Identification of anthrax toxin genes in a Bacillus cereus associated with an illness resembling inhalation anthrax
Alex R. Hoffmaster,Jacques Ravel,David A. Rasko,Gail D. Chapman,Michael D. Chute,Chung K. Marston,Barun K. De,Claudio Tavares Sacchi,Collette Fitzgerald,Leonard W. Mayer,Martin C. J. Maiden,Fergus G. Priest,Margaret Barker,Lingxia Jiang,Regina Z. Cer,Jennifer Rilstone,Scott N. Peterson,Robbin S. Weyant,Darrell R. Galloway,Timothy D. Read,Tanja Popovic,Claire M. Fraser +21 more
TL;DR: A public health approach with genome analysis is combined to provide insight into the correlation of phenotypic characteristics and their genetic basis and represents an example of how genomics could rapidly assist public health experts responding not only to clearly identified select agents but also to novel agents with similar pathogenic potentials.
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Biology and taxonomy of Bacillus cereus, Bacillus anthracis, and Bacillus thuringiensis
TL;DR: It is speculated that to understand the taxonomic relationship within this group of bacteria, special attention should be devoted also to the ecology and the population genetics of these species.
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Invertebrates as a source of emerging human pathogens
TL;DR: Examining pairs of closely related bacteria finds that one is benign, but insect associated, and one is a human pathogen, which might represent a source of new human pathogenic strains and might also foster the spread of novel virulence factors into existing human commensal or pathogenic bacteria.
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