Journal ArticleDOI
Genome sequence analysis of the temperate bacteriophage TBP2 of the solvent producer Clostridium saccharoperbutylacetonicum N1-4 (HMT, ATCC 27021).
TLDR
The genome sequence of the temperate bacteriophage TBP2 of the solventogenic strain C. saccharoperbutylacetonicum N1-4 (HMT) that is used for ABE fermentation is presented, representing the first fully sequenced genome of a temperate bacteriaiophages infecting solventogenic clostridia.Abstract:
The genus Clostridium consists of a diverse group of pathogenic and non-pathogenic bacteria The non-pathogenic clostridia contain several solventogenic members of industrial importance, such as Clostridium acetobutylicum or C beijerinckii In the process of acetone-butanol-ethanol (ABE) fermentation, these strains are used in large scale fermentation plants since almost 100 years Soon after establishment of the first plants, the fermentation processes suffered from different bacteriophage infections worldwide A limited set of studies addressing bacteriophages in solventogenic clostridia have been conducted since then In this study, we present the genome sequence of the temperate bacteriophage TBP2 of the solventogenic strain C saccharoperbutylacetonicum N1-4 (HMT) that is used for ABE fermentation The phage genome consists of 38 039 bp and includes 48 open reading frames Sequence analysis indicates that the genome encloses random parts of the bacterial genome in addition to its own DNA It represents the first fully sequenced genome of a temperate bacteriophage infecting solventogenic clostridiaread more
Citations
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SPAdes, a new genome assembly algorithm and its applications to single-cell sequencing ( 7th Annual SFAF Meeting, 2012)
TL;DR: SPAdes as mentioned in this paper is a new assembler for both single-cell and standard (multicell) assembly, and demonstrate that it improves on the recently released E+V-SC assembler and on popular assemblers Velvet and SoapDeNovo (for multicell data).
Posted ContentDOI
Renewable Fatty Acid Ester Production in Clostridium
Jun Feng,Jie Zhang,Pixiang Wang,Pablo Jiménez-Bonilla,Yanyan Gu,Junping Zhou,Mingfeng Cao,Zengyi Shao,Ilya Borovok,Yi Wang +9 more
TL;DR: The principles of selecting the most appropriate host for specific bioproduction and engineering microbial chassis to produce high-value and easy-separable endproducts are highly applicable to other bioprocesses, and could lead to breakthroughs in biofuel/biochemical production and general bioeconomy.
Journal ArticleDOI
Renewable fatty acid ester production in Clostridium.
Jun Feng,Jie Zhang,Ma Yuechao,Yiming Feng,Shangjun Wang,Na Guo,Haijiao Wang,Pixiang Wang,Pablo Jiménez-Bonilla,Yanyan Gu,Junping Zhou,Zhong Tian Zhang,Mingfend Cao,Di Jiang,Shuning Wang,Xian Wei Liu,Zengyi Shao,Ilya Borovok,Haibo Huang,Yi Wang +19 more
TL;DR: In this article, solventogenic clostridia were used for co-producing acyl-CoAs, acids and alcohols as precursors, through rational screening for host strains and enzymes, systematic metabolic engineering-including elimination of putative prophages, they developed strains that can produce 20.3
Journal ArticleDOI
Systematic strategies for developing phage resistant Escherichia coli strains
Xuan Zou,Xiaohong Xiao,Ziran Mo,Yashi Ge,Xing Jiang,Ruolin Huang,Mengxue Li,Zixin Deng,Shi Chen,Lianrong Wang,Sang Yup Lee +10 more
TL;DR: In this article , a strategy for developing phage-resistant Escherichia coli strains through the simultaneous genomic integration of a DNA phosphorothioation-based SSP defense module and mutations of components essential for the phage life cycle is presented.
References
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SPAdes, a new genome assembly algorithm and its applications to single-cell sequencing ( 7th Annual SFAF Meeting, 2012)
TL;DR: SPAdes as mentioned in this paper is a new assembler for both single-cell and standard (multicell) assembly, and demonstrate that it improves on the recently released E+V-SC assembler and on popular assemblers Velvet and SoapDeNovo (for multicell data).
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