Grapevine VlbZIP30 improves drought resistance by directly activating VvNAC17 and promoting lignin biosynthesis through the regulation of three peroxidase genes.
Mingxing Tu,Xianhang Wang,Wuchen Yin,Ya Wang,Yajuan Li,Guofeng Zhang,Zhi Li,Junyang Song,Xiping Wang +8 more
TLDR
Overexpression of VlbZIP30 improves drought tolerance, characterized by a reduction in the water loss rate, maintenance of an effective photosynthesis rate, and increased lignin content in leaves under drought conditions.Abstract:
Drought stress severely affects grapevine quality and yield, and recent reports have revealed that lignin plays an important role in protection from drought stress. Since little is known about lignin-mediated drought resistance in grapevine, we investigated its significance. Herein, we show that VlbZIP30 mediates drought resistance by activating the expression of lignin biosynthetic genes and increasing lignin deposition. Transgenic grapevine plants overexpressing VlbZIP30 exhibited lignin deposition (mainly G and S monomers) in the stem secondary xylem under control conditions, which resulted from the upregulated expression of VvPRX4 and VvPRX72. Overexpression of VlbZIP30 improves drought tolerance, characterized by a reduction in the water loss rate, maintenance of an effective photosynthesis rate, and increased lignin content (mainly G monomer) in leaves under drought conditions. Electrophoretic mobility shift assay, luciferase reporter assays, and chromatin immunoprecipitation-qPCR assays indicated that VlbZIP30 directly binds to the G-box cis-element in the promoters of lignin biosynthetic (VvPRX N1) and drought-responsive (VvNAC17) genes to regulate their expression. In summary, we report a novel VlbZIP30-mediated mechanism linking lignification and drought tolerance in grapevine. The results of this study may be of value for the development of molecular breeding strategies to produce drought-resistant fruit crops.read more
Citations
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Metabolomics and Molecular Approaches Reveal Drought Stress Tolerance in Plants.
TL;DR: In this paper, a review summarizes the most updated findings on primary and secondary metabolites involved in drought stress and discuss possible strategies to help plants to counteract unfavorable drought periods, and examine the application of useful metabolic genes and their molecular responses to drought tolerance.
Journal ArticleDOI
A Paeonia ostii caffeoyl-CoA O-methyltransferase confers drought stress tolerance by promoting lignin synthesis and ROS scavenging.
TL;DR: It is suggested that PoCCoAOMT is a vital gene in promoting lignin synthesis and ROS scavenging to confer drought stress tolerance in P. ostii.
Journal ArticleDOI
Whole-genome sequencing reveals rare off-target mutations in CRISPR/Cas9-edited grapevine
Xianhang Wang,Mingxing Tu,Ya Wang,Wuchen Yin,Yu Zhang,Hongsong Wu,Yincong Gu,Zhi Li,Zhumei Xi,Xiping Wang +9 more
TL;DR: In this paper, the authors performed whole-genome sequencing (WGS) of seven Cas9-edited grapevine plants in which one of two genes was targeted by CRISPR/Cas9 and three wild-type (WT) plants.
Journal ArticleDOI
CRISPR/Cas9-mediated mutagenesis of VvbZIP36 promotes anthocyanin accumulation in grapevine (Vitis vinifera)
Ming Zheng Tu,Jinghao Fang,Ruikang Zhao,Xinyu Liu,Wu Yin,Ya Wang,Xianhang Wang,Xiping Wang,Yulin Fang +8 more
TL;DR: In this article , a grapevine (Vitis vinifera) bZIP gene from group K, VvbZIP36, was found to act as a negative regulator of anthocyanin biosynthesis.
Journal ArticleDOI
Recent developments in cold plasma-based enzyme activity (browning, cell wall degradation, and antioxidant) in fruits and vegetables.
Sneh Punia Bangar,Monica Trif,Fatih Ozogul,Rajesh Kumar,Vandana Chaudhary,S Milan Vukic,Maharishi Tomar,Sushil Changan +7 more
TL;DR: The cold plasma technique has emerged as a novel, economic, and environmentally friendly approach that reduces the expression of ripening and browning enzymes while increasing the activity of antioxidant enzymes; microorganisms are significantly inhibited, therefore improving the shelf life of fruits and vegetables as discussed by the authors .
References
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Lignin Biosynthesis and Structure
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AREB1 Is a Transcription Activator of Novel ABRE-Dependent ABA Signaling That Enhances Drought Stress Tolerance in Arabidopsis
Yasunari Fujita,Miki Fujita,Rie Satoh,Kyonoshin Maruyama,Mohammad Masud Parvez,Motoaki Seki,Keiichiro Hiratsu,Masaru Ohme-Takagi,Kazuo Shinozaki,Kazuko Yamaguchi-Shinozaki +9 more
TL;DR: It is shown that expression of the intact AREB1 gene on its own is insufficient to lead to expression of downstream genes under normal growth conditions, and data suggest that AREB 1 regulates novel ABRE-dependent ABA signaling that enhances drought tolerance in vegetative tissues.
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Arabidopsis Basic Leucine Zipper Proteins That Mediate Stress-Responsive Abscisic Acid Signaling
TL;DR: The results provide strong in vivo evidence that ABF3 and ABF4 mediate stress-responsive ABA signaling, and the temporal and spatial expression patterns of ABF 3 and ABf4 were consistent with their suggested roles.
Journal ArticleDOI
KOBAS server: a web-based platform for automated annotation and pathway identification
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