Journal ArticleDOI
Identification of ciprofloxacin resistance by SimpleProbe™, High Resolution Melt and Pyrosequencing™ nucleic acid analysis in biothreat agents: Bacillus anthracis, Yersinia pestis and Francisella tularensis
Bonnie M. Loveless,Anastasiya Yermakova,Deanna R. Christensen,John P. Kondig,Henry S. Heine,Leonard P. Wasieloski,David A. Kulesh +6 more
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TLDR
While SimpleProbe and Pyrosequencing successfully identified all known mutants, the HRM assay identified all but those resulting from G<-->C or A<-->T substitutions.About:
This article is published in Molecular and Cellular Probes.The article was published on 2010-06-01. It has received 48 citations till now. The article focuses on the topics: Topoisomerase IV & DNA gyrase.read more
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Tularaemia: clinical aspects in Europe
Max Maurin,Miklós Gyuranecz +1 more
TL;DR: Two natural lifecycles of F tularensis have been described in this continent, although not fully characterised, which are associated with different modes of transmission, clinical features, and public health burdens of tularaemia.
Journal ArticleDOI
Detecting Biothreat Agents: From Current Diagnostics to Developing Sensor Technologies
Scott A. Walper,Guillermo Lasarte Aragonés,Guillermo Lasarte Aragonés,Kim E. Sapsford,Carl W. Brown,Carl W. Brown,Clare E. Rowland,Clare E. Rowland,Joyce C. Breger,Igor L. Medintz +9 more
TL;DR: In this paper, a review of the available methods for detecting bioagents including pathogenic bacteria and viruses along with their toxins is provided along with definitions and relevant considerations, along with an overview of the detection technologies that find use in this endeavor along with how they provide data or transduce signal within a sensing configuration.
Journal ArticleDOI
Evolution toward high-level fluoroquinolone resistance in Francisella species
Vivien Sutera,Maxime Levert,Maxime Levert,Wim P. Burmeister,Dominique Schneider,Dominique Schneider,Max Maurin,Max Maurin +7 more
TL;DR: The efficiency and speed of in vitro production of mutants highly resistant to fluoroquinolones in Francisella species emphasize the urgent need to identify all antibiotic resistance mechanisms in these species, develop molecular tools for their detection and design new therapeutic alternatives for tularaemia.
Journal ArticleDOI
Rapid Detection of Isoniazid, Rifampin and Ofloxacin Resistance in Mycobacterium tuberculosis Clinical Isolates Using High Resolution Melting Analysis
TL;DR: High-resolution melting analysis (HRMA) is a rapid, sensitive method for detection of drug resistance in M. tuberculosis which could be used routinely for screening isolates in countries with a high prevalence of tuberculosis and drug resistance or in individual isolates when drug resistance is suspected.
References
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Journal ArticleDOI
DNA gyrase, topoisomerase IV, and the 4-quinolones.
Karl Drlica,Xilin Zhao +1 more
TL;DR: Quinolone-topoisomerase biology is providing a model for understanding aspects of host-parasite interactions and providing ways to investigate manipulation of the bacterial chromosome by topoisomerases.
Journal ArticleDOI
High-Resolution Genotyping by Amplicon Melting Analysis Using LCGreen
TL;DR: High-resolution melting analysis of PCR products amplified in the presence of LCGreen can identify both heterozygous and homozygous sequence variants, and is a promising method for mutation screening.
Journal ArticleDOI
Plasmid-Mediated Quinolone Resistance: a Multifaceted Threat
TL;DR: The best-described form of PMQR is determined by the qnr group of genes, likely originating in aquatic organisms, which code for pentapeptide repeat proteins that reduce susceptibility to quinolones by protecting the complex of DNA and DNA gyrase or topoisomerase IV enzymes from the inhibitory effect of qu inolones.
Journal ArticleDOI
Quinolone resistance-determining region in the DNA gyrase gyrA gene of Escherichia coli.
TL;DR: Nucleotide sequence analysis disclosed that all nine spontaneous quinolone-resistant gyrB mutants of Escherichia coli KL16 had a point mutation from aspartic acid to asparagine at amino acid 426 and that all four type 2 mutants had apoint mutation from lysine to glutamic acid at amino acids 447.
Journal ArticleDOI
Fluoroquinolone antimicrobial agents.
John S. Wolfson,David C. Hooper +1 more
TL;DR: The fluoroquinolones offer an efficacious, well-tolerated, and cost-effective alternative to parenteral therapies of selected infections and show promise for therapy of prostatitis, respiratory tract infections, osteomyelitis, and cutaneous infections.
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