Journal ArticleDOI
Identification of two oxygenase genes involved in the respective biosynthetic pathways of canonical and non-canonical strigolactones in Lotus japonicus.
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A cytochrome P450 and a 2-oxoglutarate-dependent dioxygenase genes responsible, respectively, for the biosyntheses of canonical and non-canonical strigolactones in Lotus japonicus were identified by transcriptome profiling and mutant screening.Abstract:
A cytochrome P450 and a 2-oxoglutarate-dependent dioxygenase genes responsible, respectively, for the biosyntheses of canonical and non-canonical strigolactones in Lotus japonicus
were identified by transcriptome profiling and mutant screening. Strigolactones (SLs) are a group of apocarotenoids with diverse structures that act as phytohormones and rhizosphere signals. The model legume Lotus japonicus produces both canonical and non-canonical SLs, 5-deoxystrigol (5DS) and lotuslactone (LL), respectively, through oxidation of a common intermediate carlactone by the cytochrome P450 (CYP) enzyme MAX1. However, the pathways downstream of MAX1 and the branching point in the biosyntheses of 5DS and LL have not been elucidated. Here, we identified a CYP and a 2-oxoglutarate-dependent dioxygenase (2OGD) genes responsible, respectively, for the formation of Lotus SLs by transcriptome profiling using RNA-seq and screening of SL-deficient mutants from the Lotus retrotransposon 1 (LORE1) insertion mutant resource. The CYP and 2OGD genes were named DSD and LLD, respectively, after 5DS or LL defective phenotype of the mutants. The involvements of the genes in Lotus SL biosyntheses were confirmed by restoration of the mutant phenotype using Agrobacterium rhizogenes-mediated transformation to generate transgenic roots expressing the coding sequence. The transcript levels of DSD and LLD in roots as well as the levels of 5DS and LL in root exudates were reduced by phosphate fertilization and gibberellin treatment. This study can provide the opportunity to investigate how and why plants produce the two classes of SLs.read more
Citations
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Journal ArticleDOI
Strigolactone biosynthesis, transport and perception.
TL;DR: Recent advances in SL research are summarized with a focus on biosynthesis, transport, and perception.
Journal ArticleDOI
CYP722C from Gossypium arboreum catalyzes the conversion of carlactonoic acid to 5-deoxystrigol.
Takatoshi Wakabayashi,Kasumi Shida,Yurie Kitano,Hirosato Takikawa,Masaharu Mizutani,Yukihiro Sugimoto +5 more
TL;DR: Results strongly suggest that GaCYP722C from cotton is a 5DS synthase and that CYP7 22C is the crucial CYP subfamily involved in the generation of canonical SLs, irrespective of the different C-ring configurations.
Journal ArticleDOI
Translation of Strigolactones from Plant Hormone to Agriculture: Achievements, Future Perspectives, and Challenges.
TL;DR: How manipulation of SL signaling can be used when developing new tools and crop varieties to address some critical challenges, such as nutrient acquisition, resource allocation, stress tolerance, and plant-parasite interactions is reviewed.
Journal ArticleDOI
Recent progress in the chemistry and biochemistry of strigolactones
TL;DR: In this review, recent advances in the chemistry and biology of SLs are summarized and possible future outcomes are discussed.
Journal ArticleDOI
Hydroxyl carlactone derivatives are predominant strigolactones in Arabidopsis
Kaori Yoneyama,Kaori Yoneyama,Kohki Akiyama,Philip B. Brewer,Narumi Mori,Miyuki Kawano-Kawada,Shinsuke Haruta,Hisashi Nishiwaki,Satoshi Yamauchi,Xiaonan Xie,Mikihisa Umehara,Christine A. Beveridge,Koichi Yoneyama,Koichi Yoneyama,Takahito Nomura +14 more
TL;DR: Feed experiments with deuterated MeCLAs revealed that [MeCLA + 16 Da] is hydroxymethyl carlactonoate (1'‐HO‐MeCLA), which suggests that HO‐CL derivatives may be predominant SLs in Arabidopsis, produced through MAX1 and LBO.
References
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Journal ArticleDOI
MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets
TL;DR: The latest version of the Molecular Evolutionary Genetics Analysis (Mega) software, which contains many sophisticated methods and tools for phylogenomics and phylomedicine, has been optimized for use on 64-bit computing systems for analyzing larger datasets.
Journal ArticleDOI
Plant sesquiterpenes induce hyphal branching in arbuscular mycorrhizal fungi
TL;DR: Strigolactones are a group of sesquiterpene lactones, previously isolated as seed-germination stimulants for the parasitic weeds Striga and Orobanche, and a synthetic analogue, GR24, induced extensive hyphal branching in germinating spores of the AM fungus Gigaspora margarita at very low concentrations.
Journal ArticleDOI
The Path from β-Carotene to Carlactone, a Strigolactone-Like Plant Hormone
Adrian Alder,Muhammad Jamil,Mattia Marzorati,Mark Bruno,Martina Vermathen,Peter Bigler,Sandro Ghisla,Harro J. Bouwmeester,Peter Beyer,Salim Al-Babili +9 more
TL;DR: Knowledge of the structure of carlactone will be crucial for understanding the biology of strigolactones and may have applications in combating parasitic weeds.
Journal ArticleDOI
Strigolactones, a Novel Carotenoid-Derived Plant Hormone
TL;DR: This review focuses on SL biosynthesis, describes the hormonal and environmental factors that determine this process, and discusses SL transport and downstream signaling as well as the role of SLs in regulating plant development.
Journal ArticleDOI
Carlactone is converted to carlactonoic acid by MAX1 in Arabidopsis and its methyl ester can directly interact with AtD14 in vitro
Satoko Abe,Aika Sado,Kai Tanaka,Takaya Kisugi,Kei Asami,Saeko Ota,Hyun Il Kim,Kaori Yoneyama,Xiaonan Xie,Xiaonan Xie,Toshiyuki Ohnishi,Yoshiya Seto,Shinjiro Yamaguchi,Kohki Akiyama,Koichi Yoneyama,Koichi Yoneyama,Takahito Nomura,Takahito Nomura +17 more
TL;DR: It is shown that CL is converted into a carboxylated metabolite, named carlactonoic acid, byArabidopsis MAX1, the enzymatic function of which had been unknown, and that its methyl ester has the ability to interact with a SL receptor and suppress shoot branching in Arabidopsis.
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