Imaging through scattering microfluidic channels by digital holography for information recovery in lab on chip
TLDR
This work investigates the problem of counting and estimating velocity of cells flowing inside a scattering chip and proposes and test a method based on the recording of multiple digital holograms to retrieve improved phase-contrast images despite the strong scattering effect.Abstract:
We tackle the problem of information recovery and imaging through scattering microfluidic chips by means of digital holography (DH) In many cases the chip can become opalescent due to residual deposits settling down the inner channel faces, biofilm formation, scattering particle uptake by the channel cladding or its damaging by corrosive substances, or even by condensing effect on the exterior channels walls In these cases white-light imaging is severely degraded and no information is obtainable at all about the flowing samples Here we investigate the problem of counting and estimating velocity of cells flowing inside a scattering chip Moreover we propose and test a method based on the recording of multiple digital holograms to retrieve improved phase-contrast images despite the strong scattering effect This method helps, thanks to DH, to recover information which, otherwise, would be completely lostread more
Citations
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Review of quantitative phase-digital holographic microscopy: promising novel imaging technique to resolve neuronal network activity and identify cellular biomarkers of psychiatric disorders
Pierre Marquet,Pierre Marquet,Christian Depeursinge,Christian Depeursinge,Pierre J. Magistretti,Pierre J. Magistretti,Pierre J. Magistretti +6 more
TL;DR: Current and potential applications of QPM related to psychiatry through the identification of new and original cell biomarkers that, when combined with a range of other biomarkers, could significantly contribute to the determination of high risk developmental trajectories for psychiatric disorders are discussed.
Journal ArticleDOI
3D morphometry of red blood cells by digital holography.
Pasquale Memmolo,Lisa Miccio,Francesco Merola,Oriella Gennari,Paolo A. Netti,Paolo A. Netti,Pietro Ferraro +6 more
TL;DR: This work proves the new procedure for healthy red blood cells (RBCs) (i.e., discocytes) having a concave surface in their central region and shows that the method can be also useful to classify, in terms of morphology, different varieties of RBCs.
Journal ArticleDOI
Exploiting scattering media for exploring 3D objects
Alok Kumar Singh,Dinesh N. Naik,Dinesh N. Naik,Giancarlo Pedrini,Mitsuo Takeda,Mitsuo Takeda,Wolfgang Osten +6 more
TL;DR: This spatially incoherent lensless imaging technique is simple and capable of variable focusing with adjustable depths of focus that enables depth sensing of 3D objects that are concealed by the diffusing medium.
Journal ArticleDOI
Diagnostic Tools for Lab-on-Chip Applications Based on Coherent Imaging Microscopy
Francesco Merola,Pasquale Memmolo,Lisa Miccio,Vittorio Bianco,Melania Paturzo,Pietro Ferraro +5 more
TL;DR: Recent progresses in developing imaging tools based on coherent imaging microscopy that can be very useful when applied into biomicrofluidics are presented.
Journal ArticleDOI
Digital Holography, a metrological tool for quantitative analysis: Trends and future applications
Melania Paturzo,Vito Pagliarulo,Vittorio Bianco,Pasquale Memmolo,Lisa Miccio,Francesco Merola,Pietro Ferraro +6 more
TL;DR: It is shown that this powerful method can be a key metrological tool for the quantitative analysis and non-invasive inspection of a variety of materials, devices and processes, including the study of live biological matter and biomedical applications.
References
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