Immunohistochemical and ultrastructural visualization of different routes of oocyte elimination in adult rats.
TLDR
The results demonstrate that during oocyte elimination in adult rats the proteins involved in both processes, apoptosis and autophagy, are present in the same cell at the same time.Abstract:
Cell death is a process for maintaining homeostasis in tissues and organs. In the ovary, apoptotic cell death has been implicated in follicular atresia; in the elimination of the follicles that are not ovulated during adult life. Recent studies indicate that apoptosis and autophagy are two programmed processes of cell death. Apoptosis is performed by proteases called caspases and leads to such morphological traits as DNA fragmentation. Autophagy, in turn, is characterized by the exacerbated formation of autophagosomes; a process in which the amount of the LC3 and Lamp 1 proteins increases. In this study, oocytes from all stages of the estrous cycle of Wistar rats were analyzed. The apoptosis process was identified by immunodetecting active Caspase-3 and locating DNA fragmentation using the TUNEL technique. Autophagy was evaluated through immunodetection of the LC3 and Lamp 1 proteins, and by ultrastructural localization of autophagic vesicle formation. All techniques were conducted using the same oocytes. Results show that all phases of the estrous cycle contain dying oocytes that test positive simultaneously for apoptosis and autophagy markers. The highest level of apoptosis was found during estrus; while the proestrous stage had the highest level of autophagy. The diestrous and metestrous phases were characterized by a high frequency of the presence of markers of apoptosis and autophagy in the same oocyte. Our results demonstrate that during oocyte elimination in adult rats the proteins involved in both processes, apoptosis and autophagy, are present in the same cell at the same time.read more
Citations
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Impact of Oxidative Stress on Age-Associated Decline in Oocyte Developmental Competence.
Hiroyuki Sasaki,Toshio Hamatani,Shintaro Kamijo,Maki Iwai,Masato Kobanawa,Seiji Ogawa,Kenji Miyado,Mamoru Tanaka +7 more
TL;DR: Increased ROS and increased vulnerability of oocytes to ROS lead to spindle instability, chromosomal abnormalities, telomere shortening, and reduced developmental competence of aged oocytes, focusing on oxidative stress (OS) in oocytes.
Journal ArticleDOI
Autophagy in hypoxic ovary.
Anil K. Yadav,Pramod K. Yadav,Govind R. Chaudhary,Meenakshi Tiwari,Anumegha Gupta,Alka Sharma,Ashutosh N. Pandey,Ajai K Pandey,Shail K. Chaube +8 more
TL;DR: It is proposed that hypoxia may induce granulosa cell as well as oocyte autophagy and it could be responsible at least in part for germ cell elimination from mammalian ovary.
Journal ArticleDOI
Germ cell depletion from mammalian ovary: possible involvement of apoptosis and autophagy
Pramod K. Yadav,Meenakshi Tiwari,Anumegha Gupta,Alka Sharma,Shilpa Prasad,Ashutosh N. Pandey,Shail K. Chaube +6 more
TL;DR: It seems that apoptosis as well as autophagy could play active roles in germ cells depletion from ovary, and it is important to prevent these two pathways in order to retain the germ cells in ovary of several mammalian species that are either threatened or at the verge of extinction.
Journal ArticleDOI
IL-33 Is Required for Disposal of Unnecessary Cells during Ovarian Atresia through Regulation of Autophagy and Macrophage Migration
TL;DR: This study demonstrated that IL-33 is required for disposal of degenerative tissue during ovarian atresia using Il33−/− mice and provided direct evidence supporting an expanded role of IL- 33 in tissue integrity and aging through regulating disposal of unnecessary tissues or cells.
Journal ArticleDOI
Regulation of oogenesis in honey bee workers via programed cell death.
TL;DR: The results suggest that honey bees have co-opted a highly conserved checkpoint at mid-oogenesis to regulate the fertility of the worker caste.
References
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LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing
Yukiko Kabeya,Noboru Mizushima,Noboru Mizushima,Takashi Ueno,Akitsugu Yamamoto,Takayoshi Kirisako,Takayoshi Kirisako,Takeshi Noda,Takeshi Noda,Eiki Kominami,Yoshinori Ohsumi,Yoshinori Ohsumi,Tamotsu Yoshimori,Tamotsu Yoshimori +13 more
TL;DR: It is demonstrated that the rat microtubule‐associated protein 1 light chain 3 (LC3), a homologue of Apg8p essential for autophagy in yeast, is associated to the autophagosome membranes after processing.