In situ cell manipulation through enzymatic hydrogel photopatterning.
Katarzyna A. Mosiewicz,Laura Kolb,André J. van der Vlies,André J. van der Vlies,Mikaël M. Martino,Mikaël M. Martino,Philipp S. Lienemann,Jeffrey A. Hubbell,Martin Ehrbar,Matthias P. Lutolf +9 more
TLDR
It is shown that the 3D invasion of primary human mesenchymal stem cells can be spatiotemporally controlled by micropatterning the hydrogel with desired extracellular matrix (ECM) proteins and growth factors.Abstract:
Patterning physiologically relevant proteins in three-dimensional hydrogels without affecting the activity and stability of the proteins has been difficult. Now, by using enzymatic crosslinking reactions, in situ control over the phototriggered immobilization of virtually any desired protein in a synthetic hydrogel is demonstrated. The approach can be used to manipulate cells, as demonstrated by the three-dimensional control of the invasion of mesenchymal stem cells within poly(ethylene glycol) hydrogels.read more
Citations
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Wound repair and regeneration: Mechanisms, signaling, and translation
TL;DR: In this review, emerging concepts in tissue regeneration and repair are highlighted, and some perspectives on how to translate current knowledge into viable clinical approaches for treating patients with wound-healing pathologies are provided.
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Advancing biomaterials of human origin for tissue engineering
Fa-Ming Chen,Xiaohua Liu +1 more
TL;DR: An exploration of the structural, mechanical, biochemical and biological information present in native human tissue for bioengineering applications is focused on to provide inspiration for the design of future biomaterials.
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Progress and potential in organoid research.
TL;DR: The authors review recent progress in organoid derivation and applications and outline how advances in other disciplines might lead to more physiologically relevant organoids.
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Functional and Biomimetic Materials for Engineering of the Three-Dimensional Cell Microenvironment
Guoyou Huang,Fei Li,Xin Zhao,Yufei Ma,Yuhui Li,Min Lin,Guorui Jin,Tian Jian Lu,Guy M. Genin,Feng Xu +9 more
TL;DR: This review encapsulates where recent advances appear to leave the ever-shifting state of the art in the cell microenvironment, and it highlights areas in which substantial potential and uncertainty remain.
Journal ArticleDOI
The design of reversible hydrogels to capture extracellular matrix dynamics
TL;DR: The extracellular matrix (ECM) is a dynamic environment that constantly provides physical and chemical cues to embedded cells, and emerging trends include the use of molecular photoswitches or biomolecule hybridization to control polymer chain conformation.
References
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Journal ArticleDOI
Matrix elasticity directs stem cell lineage specification.
TL;DR: Naive mesenchymal stem cells are shown here to specify lineage and commit to phenotypes with extreme sensitivity to tissue-level elasticity, consistent with the elasticity-insensitive commitment of differentiated cell types.
Journal ArticleDOI
Synthetic biomaterials as instructive extracellular microenvironments for morphogenesis in tissue engineering
TL;DR: Although modern synthetic biomaterials represent oversimplified mimics of natural ECMs lacking the essential natural temporal and spatial complexity, a growing symbiosis of materials engineering and cell biology may ultimately result in synthetic materials that contain the necessary signals to recapitulate developmental processes in tissue- and organ-specific differentiation and morphogenesis.
Journal ArticleDOI
Designing materials for biology and medicine
Robert Langer,David A. Tirrell +1 more
TL;DR: New challenges and directions in biomaterials research are discussed, including synthetic replacements for biological tissues, designing materials for specific medical applications, and materials for new applications such as diagnostics and array technologies.
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Photodegradable Hydrogels for Dynamic Tuning of Physical and Chemical Properties
April M. Kloxin,Andrea M. Kasko,Andrea M. Kasko,Chelsea N. Salinas,Chelsea N. Salinas,Kristi S. Anseth,Kristi S. Anseth +6 more
TL;DR: A strategy to create photodegradable poly(ethylene glycol)–based hydrogels through rapid polymerization of cytocompatible macromers for remote manipulation of gel properties in situ is reported.
Journal ArticleDOI
Substrate Elasticity Regulates Skeletal Muscle Stem Cell Self-Renewal in Culture
Penney M. Gilbert,Karen Havenstrite,Klas E. G. Magnusson,Klas E. G. Magnusson,Alessandra Sacco,Nora Leonardi,Nora Leonardi,Peggy E. Kraft,N. K. Nguyen,Sebastian Thrun,Matthias P. Lutolf,Helen M. Blau +11 more
TL;DR: Using a bioengineered substrate to recapitulate key biophysical and biochemical niche features in conjunction with a highly automated single-cell tracking algorithm, it is shown that substrate elasticity is a potent regulator of MuSC fate in culture.