In Vitro 3D Cultures to Model the Tumor Microenvironment
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TLDR
For a comprehensive overview of 3D systems commonly used for studying tumor-stroma interactions, with a focus on recent advances in cancer modeling and drug discovery and testing, see as mentioned in this paper.Abstract:
It is now well established that the tumor microenvironment plays a key role in determining cancer growth, metastasis and drug resistance. Thus, it is fundamental to understand how cancer cells interact and communicate with their stroma and how this crosstalk regulates disease initiation and progression. In this setting, 3D cell cultures have gained a lot of interest in the last two decades, due to their ability to better recapitulate the complexity of tumor microenvironment and therefore to bridge the gap between 2D monolayers and animal models. Herein, we present an overview of the 3D systems commonly used for studying tumor-stroma interactions, with a focus on recent advances in cancer modeling and drug discovery and testing.read more
Citations
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Immunotherapy discovery on tumor organoid-on-a-chip platforms that recapitulate the tumor microenvironment.
TL;DR: A comprehensive review of tumor organoid-on-a-chip platforms for studying the interaction between the tumor microenvironment (TME) and the immune system is presented in this article , where different factors of the TME that recent microfluidic in vitro systems reproduce to generate advanced tools to imitate the crosstalk between TME and immune system.
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Recent Advances of Organ-on-a-Chip in Cancer Modeling Research
Xingxing Liu,Qiu-lan Su,Xiaoyu Zhang,Wen An Yang,Junhua Ning,Kangle Jia,Jinlan Xin,Huanling Li,Longfei Yu,Yuheng Liao,Diming Zhang +10 more
TL;DR: The organ-on-chip (OoC) platform, which integrates the technology of 3D cell culture, tissue engineering, and microfluidics, is emerging as a new method to simulate the critical structures of the in vivo tumor microenvironment and functional characteristics as discussed by the authors .
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Collagen Biomarkers Quantify Fibroblast Activity In Vitro and Predict Survival in Patients with Pancreatic Ductal Adenocarcinoma
TL;DR: In this article , the authors established a pseudo-3D in vitro model including humane pancreatic fibroblasts (PFs) and pancreatic cancer-associated fibroBLasts (CAFs) in combination with clinical collagen biomarkers, as a translational anti-fibrotic drug screening tool.
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Patient-specific modeling of stroma-mediated chemoresistance of pancreatic cancer using a three-dimensional organoid-fibroblast co-culture system
Sebastian Schuth,Solange Le Blanc,Teresa G Krieger,Julia Jabs,Miriam Schenk,Nathalia A. Giese,Markus W. Büchler,R. Eils,Christian Conrad,Oliver Strobel +9 more
TL;DR: In this article , a 3D co-culture of primary PDAC organoids and patient-matched CAFs was established to investigate the effect of the fibroblastic compartment on sensitivity to gemcitabine, 5fluorouracil and paclitaxel treatments using an image-based drug assay.
References
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