Journal ArticleDOI
In vitro culture and embryo metabolism of cattle and sheep embryos - a decade of achievement.
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TLDR
Compounds such as ethylenediamine tetraacetic acid (EDTA), NaN(3) and 2,4-dinitrophenol have been shown to increase embryo development and quality of resulting embryos, demonstrating that the process of ATP production is a key regulator of in vitro embryo development.About:
This article is published in Animal Reproduction Science.The article was published on 2000-07-02. It has received 199 citations till now. The article focuses on the topics: Embryo culture & Embryo.read more
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Producción in vitro de embriones bovinos: suplementación de los medios de cultivo con suero
TL;DR: Techniques for producing bovine preimplantation embryos by in vitro maturation and fertilization, offers the potential for a large number of embryos at low cost.
Journal ArticleDOI
Factors affecting in vitro embryo production: insights into dromedary camel
Adel R. Moawad,I.M. Ghoneim,I.M. Ghoneim,G. M. Darwish,Magdy R. Badr,Diya A. El-Badry,Abou Bakr A. EL-Wishy +6 more
Journal ArticleDOI
Taurina no desenvolvimento de embriões bovinos fecundados in vitro
Luiz Sérgio de Almeida Camargo,Wanderlei Ferreira de Sá,Ademir de Moraes Ferreira,João Henrique Moreira Viana,M.C.C. Araújo +4 more
TL;DR: Taurine only has a beneficial effect in the embryo development in culture medium in the absence of FCS and BSA, andfetal calf serum decreases cleavage rate, however, it improves the embryos development after the early cleavage.
Journal ArticleDOI
Effects of β-mercaptoethanol on ATP Contents in Cumulus Cell-enclosed Bovine Oocytes Matured In Vitro and Sequential Development of Resultant Embryos from In Vitro Fertilization
TL;DR: It is suggested that β-ME may increase both the numbers of cumulus cells attached to oocytes and the cell numbers of blastocysts derived from in vitro fertilization by influencing the ATP metabolism of bovine oocytes during IVM.
Journal ArticleDOI
Assisted Reproductive Techniques and Genetic Manipulation in the Common Marmoset.
Jung Eun Park,Erika Sasaki +1 more
TL;DR: A review of assisted reproductive and genetic manipulation techniques in nonhuman primate (NHP) zygotes is presented in this paper, which summarizes the recent advances in the development of assisted reproduction and GA techniques in NHP, providing the basis for the generation of genetically modified NHP disease models.
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An improved culture medium supports development of random-bred 1-cell mouse embryos in vitro
TL;DR: The in-vitro development of 1-cell embryos beyond the 2-cell stage in response to the removal of glucose and the addition of glutamine to the culture medium suggests that glucose may block some essential metabolic process, and that glutamine may be a preferred energy substrate during early development for these mouse embryos.
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Successful culture in vitro of sheep and cattle ova
TL;DR: This communication describes the successful culture of one-cell to eight-cell sheep ova and one- cell and eight- cell cattle ova to the morula and blastocyst stages and reports a high embryo survival after transfer of cultured Ova to recipient animals.
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Culture of preimplantation embryos: facts and artifacts
TL;DR: In this review, comparative information on epigenetic regulation of embryo development is discussed, including information on energy substrate and amino acid preferences of embryos and improvements in the design of culture media are discussed, so that higher yields and increased viability of embryos are achieved.
Journal ArticleDOI
Size Control in Animal Development
Ian Conlon,Martin Raff +1 more
TL;DR: This work is supported by an MRC studentship and program grant, respectively, and the authors thank C. C. and M. R. for their support.