Journal ArticleDOI
In vitro culture and embryo metabolism of cattle and sheep embryos - a decade of achievement.
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TLDR
Compounds such as ethylenediamine tetraacetic acid (EDTA), NaN(3) and 2,4-dinitrophenol have been shown to increase embryo development and quality of resulting embryos, demonstrating that the process of ATP production is a key regulator of in vitro embryo development.About:
This article is published in Animal Reproduction Science.The article was published on 2000-07-02. It has received 199 citations till now. The article focuses on the topics: Embryo culture & Embryo.read more
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Journal ArticleDOI
Differential oxidative stress induction and lethality of rat embryos after maternal exposure to t-butyl hydroperoxide during postimplantation period.
TL;DR: It is hypothesized that critical windows during early gestation may account for the differential susceptibility of developing embryos to pro-oxidants and necessitate a better understanding of this embryonic response to prooxidant exposures.
Journal ArticleDOI
Rapid cooling of rabbit embryos in a synthetic medium.
TL;DR: To conclude, Cryo3 can replace BSA in rabbit embryo rapid cooling "freezing" and warming media with a chemically defined medium with no animal-derived products: STEM ALPHA.
BookDOI
Periconception in Physiology and Medicine
Alireza Fazeli,William V. Holt +1 more
TL;DR: The first € price and the £ and $ price are net prices, subject to local VAT, and the €(D) includes 7% for Germany, the€(A) includes 10% for Austria.
Journal ArticleDOI
Improved Bovine Blastocyst Developmental Potential by L-carnitine Supplementation
Paul Christian,Ver J. Manzano,Marlon B. Ocampo,Lerma C. Ocampo,Excel Rio,S. Maylem,Nueva Ecija +6 more
TL;DR: Overall, the results demonstrated the usefulness of the procedures utilized in the maturation, fertilization and culture of bovine oocytes and early- stage embryos as indicated by a higher total cell count (improved cell activity) in L-carnitine supplementation.
Journal ArticleDOI
Absorption Spectral Analysis of Zn2+ or Cu2+ Coordination with Human Serum Albumin using Zincon
Koji Matsuura,Ikuyo Sugimoto +1 more
TL;DR: Using this method, the metals that bind to the multi-binding site of HSA, particularly Zn2+ can be evaluated by colorimetry in an ART laboratory and is potentially applicable for metal detection in HSA in ART clinics and also for quick analysis of the coordination of 64Cu2+ with HSA as a probe chemical in positron emission tomography.
References
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Journal ArticleDOI
An improved culture medium supports development of random-bred 1-cell mouse embryos in vitro
TL;DR: The in-vitro development of 1-cell embryos beyond the 2-cell stage in response to the removal of glucose and the addition of glutamine to the culture medium suggests that glucose may block some essential metabolic process, and that glutamine may be a preferred energy substrate during early development for these mouse embryos.
Journal ArticleDOI
Successful culture in vitro of sheep and cattle ova
TL;DR: This communication describes the successful culture of one-cell to eight-cell sheep ova and one- cell and eight- cell cattle ova to the morula and blastocyst stages and reports a high embryo survival after transfer of cultured Ova to recipient animals.
Journal ArticleDOI
Culture of preimplantation embryos: facts and artifacts
TL;DR: In this review, comparative information on epigenetic regulation of embryo development is discussed, including information on energy substrate and amino acid preferences of embryos and improvements in the design of culture media are discussed, so that higher yields and increased viability of embryos are achieved.
Journal ArticleDOI
Size Control in Animal Development
Ian Conlon,Martin Raff +1 more
TL;DR: This work is supported by an MRC studentship and program grant, respectively, and the authors thank C. C. and M. R. for their support.