Journal ArticleDOI
In vitro culture and embryo metabolism of cattle and sheep embryos - a decade of achievement.
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TLDR
Compounds such as ethylenediamine tetraacetic acid (EDTA), NaN(3) and 2,4-dinitrophenol have been shown to increase embryo development and quality of resulting embryos, demonstrating that the process of ATP production is a key regulator of in vitro embryo development.About:
This article is published in Animal Reproduction Science.The article was published on 2000-07-02. It has received 199 citations till now. The article focuses on the topics: Embryo culture & Embryo.read more
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Effects of porcine granulocyte-macrophage colony-stimulating factor on porcine in vitro-fertilized embryos.
TL;DR: PGM-CSF may have a physiological role in promoting the development of porcine preimplantation embryos and regulating cell viability and that addition of pGM- CSF to IVC medium at Days 4 to 7 or 0 to 7 improves the developmental potential of porCine IVF embryos.
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Spatial differences in gene expression in the bovine oviduct
V. Maillo,Celia de Frutos,Peadar O'Gaora,Niamh Forde,Gregory W. Burns,Thomas E. Spencer,Alfonso Gutiérrez-Adán,Patrick Lonergan,Dimitrios Rizos +8 more
TL;DR: Gene ontology revealed that the main biological processes overrepresented in the isthmus were synthesis of nitrogen, lipids, nucleotides, steroids and cholesterol as well as vesicle-mediated transport, cell cycle, apoptosis, endocytosis and exocythesis, whereas cell motion, motility and migration, DNA repair, calcium ion homeostasis, carbohydrate biosynthesis, and regulation of cilium movement and beat frequency were over represented in the ampulla.
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In vitro production of bovine embryos in medium supplemented with a serum replacer: effects on blastocyst development, cryotolerance and survival to term.
K. Moore,C.J. Rodriguez-Sallaberry,J.M. Kramer,S. Johnson,Ewa Wroclawska,S. L. Goicoa,Amir Niasari-Naslaji +6 more
TL;DR: In conclusion, the use of SR for maturation of oocytes was not beneficial; however, SR enhanced embryo culture by improving development in vitro, cryotolerance and survival, effectively replacing serum in culture.
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Leptin has concentration and stage-dependent effects on embryonic development in vitro
TL;DR: The effect of exogenous leptin on embryo development was established and the concentration-dependent regulation pattern was confirmed using sheep embryos, under similar conditions although sheep embryos appeared to be more sensitive in responding to leptin.
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Transcriptomic signature to oxidative stress exposure at the time of embryonic genome activation in bovine blastocysts.
Gael Cagnone,Marc-André Sirard +1 more
TL;DR: Results illustrate how oxidative disruption of pre‐attachment development is associated with systematic up‐regulation of several metabolic markers and indicates that a better capacity to survive anti‐oxidant depletion may allow for the survival of blastocysts with a quieter metabolism after compaction.
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An improved culture medium supports development of random-bred 1-cell mouse embryos in vitro
TL;DR: The in-vitro development of 1-cell embryos beyond the 2-cell stage in response to the removal of glucose and the addition of glutamine to the culture medium suggests that glucose may block some essential metabolic process, and that glutamine may be a preferred energy substrate during early development for these mouse embryos.
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Successful culture in vitro of sheep and cattle ova
TL;DR: This communication describes the successful culture of one-cell to eight-cell sheep ova and one- cell and eight- cell cattle ova to the morula and blastocyst stages and reports a high embryo survival after transfer of cultured Ova to recipient animals.
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Culture of preimplantation embryos: facts and artifacts
TL;DR: In this review, comparative information on epigenetic regulation of embryo development is discussed, including information on energy substrate and amino acid preferences of embryos and improvements in the design of culture media are discussed, so that higher yields and increased viability of embryos are achieved.
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Size Control in Animal Development
Ian Conlon,Martin Raff +1 more
TL;DR: This work is supported by an MRC studentship and program grant, respectively, and the authors thank C. C. and M. R. for their support.