Journal ArticleDOI
In vitro culture and embryo metabolism of cattle and sheep embryos - a decade of achievement.
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TLDR
Compounds such as ethylenediamine tetraacetic acid (EDTA), NaN(3) and 2,4-dinitrophenol have been shown to increase embryo development and quality of resulting embryos, demonstrating that the process of ATP production is a key regulator of in vitro embryo development.About:
This article is published in Animal Reproduction Science.The article was published on 2000-07-02. It has received 199 citations till now. The article focuses on the topics: Embryo culture & Embryo.read more
Citations
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Journal ArticleDOI
Current status of embryo technologies in sheep and goat.
TL;DR: The recent improvements of embryo production and freezing technologies could be used for constitution of flocks without risks of disease transmission and will allow wider propagation of valuable genes in small ruminants populations in the future.
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Influence of in vitro systems on embryo survival and fetal development in cattle.
TL;DR: Findings indicate that exposure to some in vitro environments during the first 7 days of life can profoundly influence fetal and placental development in cattle.
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Effect of culture environment on gene expression and developmental characteristics in IVF-derived embryos
TL;DR: Current literature is reviewed, with emphasis on the bovine model, demonstrating evidence for an effect of post fertilization culture environment on embryo gene expression and quality.
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Embryo culture medium: which is the best?
Michelle Lane,David K. Gardner +1 more
TL;DR: It is prudent to not only examine the ability of a culture system to produce a pregnancy with the one or two highest-grade embryos, but also to determine how many embryos from the entire cohort (both fresh and frozen embryos) are capable of producing a live birth.
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Somatic cell nuclear transfer: Past, present and future perspectives.
Keith H.S. Campbell,Pat Fisher,Wenchao Chen,Inchul Choi,Richard Kelly,Joon-Hee Lee,Joon-Hee Lee,J Xhu +7 more
TL;DR: The process of animal production by nuclear transfer is discussed and in particular changes in the methodology which have increased development and survival, simplified or increased robustness of the technique are discussed.
References
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Journal ArticleDOI
Pregnancy rates and births after unilateral or bilateral transfer of bovine embryos produced in vitro.
TL;DR: Late morulae and blastocysts produced in vitro were nonsurgically transferred to heifers by unilateral or bilateral transfer and the pregnancy rate was 54% with embryos evaluated as morphologically excellent or good, 51% with fair embryos and 26% with poor ones.
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Modifications made to culture medium by bovine oviduct epithelial cells: Changes to carbohydrates stimulate bovine embryo development
TL;DR: Results from this study demonstrate that BOE cells create an environment favourable to embryonic development, and modifications made to carbohydrate levels in synthetic oviduct fluid medium and their effect on blastocyst development in vitro assessed.
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In vitro culture of bovine IVM-IVF embryos: Cooperative interaction among embryos and the role of growth factors
TL;DR: Bovine embryos cultured in groups had a significantly higher rate of development to the blastocyst stage than embryos cultured singly, and epidermal growth factor stimulated hatching of embryos cultured singingly from the 8-cell stage, but did not significantly affect Blastocyst development.
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Effect of hyaluronic acid on development of in vitro produced bovine embryos.
TL;DR: It is concluded that supplementation of both semi-defined and defined culture media with 1 mg/mL HA improves the development of IVM/IVF bovine embryos to the blastocyst stage, without affecting embryo quality and post-freeze survival.
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Determination of pentose phosphate and Embden-Meyerhof pathway activities in bovine embryos.
M.H. Javed,Raymond W. Wright +1 more
TL;DR: In good quality bovine embryos total glucose utilization is low until 16-cell stage, with PPP being the predominant pathway, and PPP activity increases significantly in poor quality embryos and in embryos 24 h in culture.