Journal ArticleDOI
In vitro culture and embryo metabolism of cattle and sheep embryos - a decade of achievement.
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TLDR
Compounds such as ethylenediamine tetraacetic acid (EDTA), NaN(3) and 2,4-dinitrophenol have been shown to increase embryo development and quality of resulting embryos, demonstrating that the process of ATP production is a key regulator of in vitro embryo development.About:
This article is published in Animal Reproduction Science.The article was published on 2000-07-02. It has received 199 citations till now. The article focuses on the topics: Embryo culture & Embryo.read more
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Journal ArticleDOI
Selenium improves the developmental ability and reduces the apoptosis in porcine parthenotes.
TL;DR: It is suggested that SS improves the development rate and quality of porcine parthenotes by preventing oxidative damage and apoptosis.
Journal ArticleDOI
Relationship Between Development, Metabolism, and Mitochondrial Organization in 2-Cell Hamster Embryos in the Presence of Low Levels of Phosphate
TL;DR: It is shown that, for hamster embryos, inclusion of even exceptionally low concentrations of Pi in culture medium dramatically alters embryo physiology, and although 2-cell embryos can tolerate some structural disruption without concomitant, detrimental effects on development or metabolic activity, metabolic disturbance is associated with decreased developmental competence.
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Respiration rates correlate with mRNA expression of G6PD and GLUT1 genes in individual bovine in vitro-produced blastocysts
A. S. Lopes,Christine Wrenzycki,N. B. Ramsing,Doris Herrmann,Heiner Niemann,Peter Løvendahl,Torben Greve,Henrik Callesen +7 more
TL;DR: Investigation of the relationship between respiration rates and relative abundances of G6PD and GLUT1 transcripts in individual bovine blastocysts produced in vitro suggest that expression of genes for oxidative phosphorylation and glycolysis are both involved in oxygen demanding ATP production.
Journal ArticleDOI
Metabolic regulation of in vitro-produced bovine embryos. II. Effects of phenazine ethosulfate, sodium azide and 2,4-dinitrophenol during post-compaction development on glucose metabolism and lipid accumulation.
TL;DR: The PES treatment increased glucose metabolism, tended to increase the PPP flux of glucose and clearly reduced accumulation of lipids in embryos produced in the chemically defined media used.
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The impact of nutrition of the cumulus oocyte complex and embryo on subsequent development in ruminants.
TL;DR: The laboratory has shown that during in vitro culture, too little glucose during cumulus-oocyte complex maturation affects meiotic competence, and the implication of these examples to the fundamental concept of peri-conception nutritional programming of development are discussed.
References
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Journal ArticleDOI
An improved culture medium supports development of random-bred 1-cell mouse embryos in vitro
TL;DR: The in-vitro development of 1-cell embryos beyond the 2-cell stage in response to the removal of glucose and the addition of glutamine to the culture medium suggests that glucose may block some essential metabolic process, and that glutamine may be a preferred energy substrate during early development for these mouse embryos.
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Successful culture in vitro of sheep and cattle ova
TL;DR: This communication describes the successful culture of one-cell to eight-cell sheep ova and one- cell and eight- cell cattle ova to the morula and blastocyst stages and reports a high embryo survival after transfer of cultured Ova to recipient animals.
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Culture of preimplantation embryos: facts and artifacts
TL;DR: In this review, comparative information on epigenetic regulation of embryo development is discussed, including information on energy substrate and amino acid preferences of embryos and improvements in the design of culture media are discussed, so that higher yields and increased viability of embryos are achieved.
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Size Control in Animal Development
Ian Conlon,Martin Raff +1 more
TL;DR: This work is supported by an MRC studentship and program grant, respectively, and the authors thank C. C. and M. R. for their support.