In vivo imaging of EVs in zebrafish: New perspectives from "the waterside".
Vincenzo Verdi,Anaïs Bécot,Guillaume van Niel,Frederik J. Verweij +3 more
- Vol. 3, Iss: 11, pp 918-929
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TLDR
The state of the art of EV imaging in vivo is analyzed, focusing in particular on zebrafish as a promising model to visualize, study, and characterize endogenous EVs in real‐time and expand the understanding of EV biology at cellular and systems level.Abstract:
To harmoniously coordinate the activities of all its different cell types, a multicellular organism critically depends on intercellular communication. One recently discovered mode of intercellular cross-talk is based on the exchange of "extracellular vesicles" (EVs). EVs are nano-sized heterogeneous lipid bilayer vesicles enriched in a variety of biomolecules that mediate short- and long-distance communication between different cells, and between cells and their environment. Numerous studies have demonstrated important aspects pertaining to the dynamics of their release, their uptake, and sub-cellular fate and roles in vitro. However, to demonstrate these and other aspects of EV biology in a relevant, fully physiological context in vivo remains challenging. In this review we analyze the state of the art of EV imaging in vivo, focusing in particular on zebrafish as a promising model to visualize, study, and characterize endogenous EVs in real-time and expand our understanding of EV biology at cellular and systems level.read more
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Quantifying exosome secretion from single cells reveals a modulatory role for GPCR signaling (vol 217, pg 1129, 2018)
Frederik J. Verweij,Maarten P. Bebelman,Connie R. Jimenez,Juan J. Garcia-Vallejo,Hans Janssen,Jacques Neefjes,Jaco C. Knol,Richard de Goeij-de Haas,Sander R. Piersma,S. Rubina Baglio,Matthijs Verhage,Jaap M. Middeldorp,Anoek Zomer,Jacco van Rheenen,Marc G. Coppolino,Ilse Hurbain,Graça Raposo,Martine J. Smit,Ruud F. Toonen,Guillaume van Niel,D. Michiel Pegtel +20 more
TL;DR: This study designs tetraspanin-based pH-sensitive optical reporters that detect MVB–PM fusion using live total internal reflection fluorescence and dynamic correlative light–electron microscopy and highlights the modulatory dynamics of MVB exocytosis that will help to increase the understanding of exosome physiology and identify druggable targets in exosomal pathologies.
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Migrasomes: From Biogenesis, Release, Uptake, Rupture to Homeostasis and Diseases
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Center–periphery structure in research communities
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Recent advances in the crosstalk between adipose, muscle and bone tissues in fish
Isabelle Hue,Encarnación Capilla,Enrique Rosell-Moll,Sara Balbuena-Pecino,Valentine Goffette,Jean-Charles Gabillard,Isabel Navarro +6 more
TL;DR: In this article , the authors presented their state of the art focusing on local actions and inter-tissue effects of cytokines in fish, including adipokines, myokines and osteokines.
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Extracellular Vesicles and Intercellular Communication: Challenges for In Vivo Molecular Imaging and Tracking
Debora Petroni,Costanza Fabbri,Serena Babboni,Luca Menichetti,Giuseppina Basta,Serena Del Turco +5 more
TL;DR: Extracellular Vesicles (EVs) are a heterogeneous class of cell-derived membrane vesicles released by various cell types that serve as mediators of intercellular signaling as discussed by the authors .
References
More filters
Journal ArticleDOI
Versatile genome editing.
TL;DR: Prime editors enable different types of small mutations to be introduced into genomes to facilitate the introduction of new types of mutations into genomes.
Journal ArticleDOI
Putting Cells into Context.
TL;DR: This work focuses on a rather simple but often overlooked property that provides tissues with their rigidity or elasticity, a feature of cells embedded in extracellular matrix (ECM) with diverse but also organ-specific properties.