In vivo imaging of EVs in zebrafish: New perspectives from "the waterside".
Vincenzo Verdi,Anaïs Bécot,Guillaume van Niel,Frederik J. Verweij +3 more
- Vol. 3, Iss: 11, pp 918-929
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TLDR
The state of the art of EV imaging in vivo is analyzed, focusing in particular on zebrafish as a promising model to visualize, study, and characterize endogenous EVs in real‐time and expand the understanding of EV biology at cellular and systems level.Abstract:
To harmoniously coordinate the activities of all its different cell types, a multicellular organism critically depends on intercellular communication. One recently discovered mode of intercellular cross-talk is based on the exchange of "extracellular vesicles" (EVs). EVs are nano-sized heterogeneous lipid bilayer vesicles enriched in a variety of biomolecules that mediate short- and long-distance communication between different cells, and between cells and their environment. Numerous studies have demonstrated important aspects pertaining to the dynamics of their release, their uptake, and sub-cellular fate and roles in vitro. However, to demonstrate these and other aspects of EV biology in a relevant, fully physiological context in vivo remains challenging. In this review we analyze the state of the art of EV imaging in vivo, focusing in particular on zebrafish as a promising model to visualize, study, and characterize endogenous EVs in real-time and expand our understanding of EV biology at cellular and systems level.read more
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Engineered extracellular vesicles (EVs): Promising diagnostic/therapeutic tools for pediatric high-grade glioma.
Yuan-Yuan Lyu,Yupei Guo,Chioma M. Okeoma,Zhaoyue Yan,Nan Hu,Zian Li,Shaolong Zhou,Xin Zhao,Junqi Li,Xinjun Wang +9 more
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Center-Periphery Structure in Communities: Extracellular Vesicles.
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Journal ArticleDOI
Melanoma exosomes educate bone marrow progenitor cells toward a pro-metastatic phenotype through MET
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TL;DR: The data show that exosome production, transfer and education of bone marrow cells supports tumor growth and metastasis, has prognostic value and offers promise for new therapeutic directions in the metastatic process.
Journal ArticleDOI
Reassessment of Exosome Composition
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TL;DR: High-resolution density gradient fractionation and direct immunoaffinity capture are employed to precisely characterize the RNA, DNA, and protein constituents of exosomes and other non-vesicle material and show that small extracellular vesicles are not vehicles of active DNA release.