In vivo three-photon microscopy of subcortical structures within an intact mouse brain
Nicholas G. Horton,Ke Wang,Demirhan Kobat,Catharine G. Clark,Frank W. Wise,Chris B. Schaffer,Chris Xu +6 more
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TLDR
Non-invasive, high-resolution, in vivo imaging of subcortical structures (the external capsule and hippocampus) within an intact mouse brain is demonstrated using three-photon fluorescence microscopy at the new spectral window of 1700 nm.Abstract:
Two-photon fluorescence microscopy (2PM)1 enables scientists in various fields including neuroscience2,3, embryology4, and oncology5 to visualize in vivo and ex vivo tissue morphology and physiology at a cellular level deep within scattering tissue. However, tissue scattering limits the maximum imaging depth of 2PM within the mouse brain to the cortical layer, and imaging subcortical structures currently requires the removal of overlying brain tissue3 or the insertion of optical probes6,7. Here we demonstrate non-invasive, high resolution, in vivo imaging of subcortical structures within an intact mouse brain using three-photon fluorescence microscopy (3PM) at a spectral excitation window of 1,700 nm. Vascular structures as well as red fluorescent protein (RFP)-labeled neurons within the mouse hippocampus are imaged. The combination of the long excitation wavelength and the higher order nonlinear excitation overcomes the limitations of 2PM, enabling biological investigations to take place at greater depth within tissue.read more
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Near-infrared fluorophores for biomedical imaging
TL;DR: This Review covers recent progress on near-infrared fluorescence imaging for preclinical animal studies and clinical diagnostics and interventions.
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Carbon Nanomaterials for Biological Imaging and Nanomedicinal Therapy
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Through-skull fluorescence imaging of the brain in a new near-infrared window
Guosong Hong,Shuo Diao,Junlei Chang,Alexander L. Antaris,Changxin Chen,Bo Zhang,Su Zhao,Dmitriy N. Atochin,Paul L. Huang,Katrin I. Andreasson,Calvin J. Kuo,Hongjie Dai +11 more
TL;DR: Through-scalp and through-skull fluorescence imaging of mouse cerebral vasculature without craniotomy is reported utilizing the intrinsic photoluminescence of single-walled carbon nanotubes in the 1.3–1.4 micrometre near-infrared window, providing real-time assessment of blood flow anomaly in a mouse middle cerebral artery occlusion stroke model.
Journal ArticleDOI
Sensitive red protein calcium indicators for imaging neural activity
Hod Dana,Boaz Mohar,Boaz Mohar,Yi Sun,Sujatha Narayan,Andrew Gordus,Jeremy P. Hasseman,Getahun Tsegaye,Graham T Holt,Amy Hu,Deepika Walpita,Ronak Patel,John J. Macklin,Cornelia I. Bargmann,Misha B. Ahrens,Eric R. Schreiter,Vivek Jayaraman,Loren L. Looger,Karel Svoboda,Douglas S. Kim +19 more
TL;DR: Improved red GECIs based on mRuby (jRCaMP1a, b) and mApple (jRGECO1a) are presented, with sensitivity comparable to GCaMP6, to facilitate deep-tissue imaging, dual-color imaging together with GFP-based reporters, and the use of optogenetics in combination with calcium imaging.
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Swept confocally-aligned planar excitation (SCAPE) microscopy for high speed volumetric imaging of behaving organisms.
Matthew B. Bouchard,Venkatakaushik Voleti,César S. Mendes,Clay Lacefield,Wesley B. Grueber,Richard S. Mann,Randy M. Bruno,Elizabeth M. C. Hillman +7 more
TL;DR: A new 3D microscopy technique that allows volumetric imaging of living samples at ultra-high speeds: Swept, confocally-aligned planar excitation (SCAPE) microscopy, demonstrated by imaging spontaneous neuronal firing in the intact brain of awake behaving mice, as well as freely moving transgenic Drosophila larvae.
References
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