Islet cyclic AMP levels are not lowered during alpha 2-adrenergic inhibition of insulin release.
S Ullrich,C B Wollheim +1 more
TLDR
It is suggested that epinephrine inhibits insulin release at a step distal to the generation of cyclic AMP.About:
This article is published in Journal of Biological Chemistry.The article was published on 1984-04-10 and is currently open access. It has received 98 citations till now. The article focuses on the topics: Glycogenesis & Forskolin.read more
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Receptors linked to inhibition of adenylate cyclase: additional signaling mechanisms.
TL;DR: This review summarizes the current understanding of how these receptors are coupled to their multiple potential effector mechanisms and offers some speculation about the possible interplay between the biochemical and electrophysiological sequels of receptor occupancy.
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Simultaneous coupling of alpha 2-adrenergic receptors to two G-proteins with opposing effects. Subtype-selective coupling of alpha 2C10, alpha 2C4, and alpha 2C2 adrenergic receptors to Gi and Gs.
TL;DR: It is observed that in the presence of low concentrations of the alpha 2AR agonist UK-14304, alpha 2C10 mediated inhibition of adenylyl cyclase activity, whereas at high concentrations of agonist,alpha 2C 10 mediated stimulation of adencylylcyclase activity.
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Guanine nucleotides induce Ca2+-independent insulin secretion from permeabilized RINm5F cells.
TL;DR: The findings point to a guanine nucleotide-regulated site in the activation of insulin secretion different from the known transmembrane signalling systems.
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Isolation of rat genomic clones encoding subtypes of the alpha 2-adrenergic receptor. Identification of a unique receptor subtype.
TL;DR: Data suggest that RG20 represents a fourth alpha 2-AR subtype distinct from the known alpha 2A-C receptor subtypes, which is also capable of mediating adenylylcyclase inhibition as determined following its stable expression in NIH-3T3 fibroblasts.
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Serotonin as a growth factor.
Klaus Seuwen,Jacques Pouysségur +1 more
TL;DR: Cette revue considere les donnees recentes concernant l'activite mitogenique de la serotonine and le role of ce neurotransmetteur en tant que facteur de croissance.
References
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Coated charcoal immunoassay of insulin.
TL;DR: The procedure here described yields a straight line graph when insulin added is plotted against insulin recovered, and makes simpler and more rapid the immunoassay of insulin in biologic fluids, using radioisotope dilution with 131I-insulin and "biopsy" of the insulin pool by antibody to insulin.
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Method for the Isolation of Intact Islets of Langerhans from the Rat Pancreas
Paul E. Lacy,Mery Kostianovsky +1 more
TL;DR: A simple method for the isolation of intact islets from the normal rat pancreas is described, based upon disruption of the acinar parenchyma by injecting Hanks solution into the pancreatic duct system followed by incubation of the Pancreas in collagenase.
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The role of hormone receptors and GTP-regulatory proteins in membrane transduction
TL;DR: Cell membrane receptors for hormones and neurotransmitters form oligomeric complexes with GTP-regulatory proteins and inhibit the latter from reacting with G TP, and this theory may apply generally to membrane signal transduction involving surface receptors.
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Forskolin: unique diterpene activator of adenylate cyclase in membranes and in intact cells.
TL;DR: F Forskolin would appear to activate adenylate cyclase through a unique mechanism involving both direct activation of the enzyme and facilitation or potentiation of the modulation of enzyme activity by receptors or the guanyl nucleotide-binding subunit, or both.
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The stimulus-secretion coupling of glucose-induced insulin release. 3. Uptake of 45 calcium by isolated islets of Langerhans.
TL;DR: In this article, the role of calcium in glucose-induced insulin secretion was examined by measuring 45Ca uptake by isolated islets of Langerhans, which were incubated for 90 min in the presence of 45Ca, submitted to successive washings, and finally examined for their radioactive content.